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Proteomic responses to metal-induced oxidative stress in hydrothermal vent-living mussels, Bathymodiolus sp., on the Southwest Indian Ridge

Proteomic responses to metal-induced oxidative stress in hydrothermal vent-living mussels, Bathymodiolus sp., on the Southwest Indian Ridge
Proteomic responses to metal-induced oxidative stress in hydrothermal vent-living mussels, Bathymodiolus sp., on the Southwest Indian Ridge
Bathymodiolin mussels are amongst the dominant fauna occupying hydrothermal vent ecosystems throughout the World's oceans. This subfamily inhabits a highly ephemeral and variable environment, where exceptionally high concentrations of reduced sulphur species and heavy metals necessitate adaptation of specialised detoxification mechanisms. Whilst cellular responses to common anthropogenic pollutants are well-studied in shallow-water species, they remain limited in deep-sea vent fauna. Bathymodiolus sp. were sampled from two newly-discovered vent sites on the Southwest Indian Ridge (Tiamat and Knuckers Gaff) by the remotely operated vehicle (ROV) Kiel 6000 during the RRS James Cook cruise, JC 067 in November 2011. Here, we use redox proteomics to investigate the effects of tissue metal accumulation on protein expression and thiol oxidation in gill. Following 2D PAGE, we demonstrate a significant difference in intensity in 30 protein spots in this organ between the two vent sites out of 205 matched spots. We also see significant variations in thiol oxidation in 15 spots, out of 143 matched. At Tiamat, 23 protein spots are up-regulated compared to Knuckers Gaff and we identify 5 of these with important roles in metabolism, cell structure, stress response, and redox homeostasis. We suggest that increased metal exposure triggers changes in the proteome, regulating tissue uptake. This is evident both between vent sites and across a chemical gradient within the Knuckers Gaff vent site. Our findings highlight the importance of proteomic plasticity in successful adaptation to the spatially and temporally fluctuating chemical environments that are characteristic of hydrothermal vent habitats.
hydrothermal activity, southwest indian ridge, bathymodiolus sp., metals, bioaccumulation, oxidative stress, detoxification, proteome
0141-1136
29-37
Cole, Catherine
3e751bbf-d25a-4e86-9eac-f7c0a0d5033d
Coelho, Ana Varela
182203bf-6fdd-41f2-82a9-cc663143dc4f
James, Rachael H.
79aa1d5c-675d-4ba3-85be-fb20798c02f4
Connelly, Doug
d49131bb-af38-4768-9953-7ae0b43e33c8
Sheehan, David
fca00c9c-6872-40bd-b840-a4c9d3b8f96d
Cole, Catherine
3e751bbf-d25a-4e86-9eac-f7c0a0d5033d
Coelho, Ana Varela
182203bf-6fdd-41f2-82a9-cc663143dc4f
James, Rachael H.
79aa1d5c-675d-4ba3-85be-fb20798c02f4
Connelly, Doug
d49131bb-af38-4768-9953-7ae0b43e33c8
Sheehan, David
fca00c9c-6872-40bd-b840-a4c9d3b8f96d

Cole, Catherine, Coelho, Ana Varela, James, Rachael H., Connelly, Doug and Sheehan, David (2014) Proteomic responses to metal-induced oxidative stress in hydrothermal vent-living mussels, Bathymodiolus sp., on the Southwest Indian Ridge. [in special issue: Pollutant Responses in Marine Organisms (PRIMO17)] Marine Environmental Research, 96, 29-37. (doi:10.1016/j.marenvres.2013.09.003).

Record type: Article

Abstract

Bathymodiolin mussels are amongst the dominant fauna occupying hydrothermal vent ecosystems throughout the World's oceans. This subfamily inhabits a highly ephemeral and variable environment, where exceptionally high concentrations of reduced sulphur species and heavy metals necessitate adaptation of specialised detoxification mechanisms. Whilst cellular responses to common anthropogenic pollutants are well-studied in shallow-water species, they remain limited in deep-sea vent fauna. Bathymodiolus sp. were sampled from two newly-discovered vent sites on the Southwest Indian Ridge (Tiamat and Knuckers Gaff) by the remotely operated vehicle (ROV) Kiel 6000 during the RRS James Cook cruise, JC 067 in November 2011. Here, we use redox proteomics to investigate the effects of tissue metal accumulation on protein expression and thiol oxidation in gill. Following 2D PAGE, we demonstrate a significant difference in intensity in 30 protein spots in this organ between the two vent sites out of 205 matched spots. We also see significant variations in thiol oxidation in 15 spots, out of 143 matched. At Tiamat, 23 protein spots are up-regulated compared to Knuckers Gaff and we identify 5 of these with important roles in metabolism, cell structure, stress response, and redox homeostasis. We suggest that increased metal exposure triggers changes in the proteome, regulating tissue uptake. This is evident both between vent sites and across a chemical gradient within the Knuckers Gaff vent site. Our findings highlight the importance of proteomic plasticity in successful adaptation to the spatially and temporally fluctuating chemical environments that are characteristic of hydrothermal vent habitats.

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Cole et al., 2014, Mar Environ Res.pdf - Accepted Manuscript
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Published date: May 2014
Keywords: hydrothermal activity, southwest indian ridge, bathymodiolus sp., metals, bioaccumulation, oxidative stress, detoxification, proteome
Organisations: Geochemistry, Marine Geoscience

Identifiers

Local EPrints ID: 362920
URI: http://eprints.soton.ac.uk/id/eprint/362920
ISSN: 0141-1136
PURE UUID: 9ac6ddb1-35f2-4cfb-aadc-370b93e9dc91
ORCID for Rachael H. James: ORCID iD orcid.org/0000-0001-7402-2315

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Date deposited: 10 Mar 2014 10:52
Last modified: 15 Mar 2024 03:30

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Contributors

Author: Catherine Cole
Author: Ana Varela Coelho
Author: Doug Connelly
Author: David Sheehan

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