The University of Southampton
University of Southampton Institutional Repository

Sequence-specific recognition of DNA nanostructures

Sequence-specific recognition of DNA nanostructures
Sequence-specific recognition of DNA nanostructures
DNA is the most exploited biopolymer for the programmed self-assembly of objects and devices that exhibit nanoscale-sized features. One of the most useful properties of DNA nanostructures is their ability to be functionalized with additional non-nucleic acid components. The introduction of such a component is often achieved by attaching it to an oligonucleotide that is part of the nanostructure, or hybridizing it to single-stranded overhangs that extend beyond or above the nanostructure surface. However, restrictions in nanostructure design and/or the self-assembly process can limit the suitability of these procedures. An alternative strategy is to couple the component to a DNA recognition agent that is capable of binding to duplex sequences within the nanostructure. This offers the advantage that it requires little, if any, alteration to the nanostructure and can be achieved after structure assembly. In addition, since the molecular recognition of DNA can be controlled by varying pH and ionic conditions, such systems offer tunable properties that are distinct from simple Watson–Crick hybridization. Here, we describe methodology that has been used to exploit and characterize the sequence-specific recognition of DNA nanostructures, with the aim of generating functional assemblies for bionanotechnology and synthetic biology applications.

dna nanostructure, self-assembly, crossover
1046-2023
1-11
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Rusling, David A. and Fox, Keith R. (2014) Sequence-specific recognition of DNA nanostructures. Methods, 1-11. (doi:10.1016/j.ymeth.2014.02.028).

Record type: Article

Abstract

DNA is the most exploited biopolymer for the programmed self-assembly of objects and devices that exhibit nanoscale-sized features. One of the most useful properties of DNA nanostructures is their ability to be functionalized with additional non-nucleic acid components. The introduction of such a component is often achieved by attaching it to an oligonucleotide that is part of the nanostructure, or hybridizing it to single-stranded overhangs that extend beyond or above the nanostructure surface. However, restrictions in nanostructure design and/or the self-assembly process can limit the suitability of these procedures. An alternative strategy is to couple the component to a DNA recognition agent that is capable of binding to duplex sequences within the nanostructure. This offers the advantage that it requires little, if any, alteration to the nanostructure and can be achieved after structure assembly. In addition, since the molecular recognition of DNA can be controlled by varying pH and ionic conditions, such systems offer tunable properties that are distinct from simple Watson–Crick hybridization. Here, we describe methodology that has been used to exploit and characterize the sequence-specific recognition of DNA nanostructures, with the aim of generating functional assemblies for bionanotechnology and synthetic biology applications.

This record has no associated files available for download.

More information

Published date: 28 February 2014
Keywords: dna nanostructure, self-assembly, crossover
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 363326
URI: http://eprints.soton.ac.uk/id/eprint/363326
ISSN: 1046-2023
PURE UUID: 5773f778-5153-41b7-9d23-c3468f0da164
ORCID for David A. Rusling: ORCID iD orcid.org/0000-0002-7442-686X
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 24 Mar 2014 13:27
Last modified: 15 Mar 2024 02:36

Export record

Altmetrics

Contributors

Author: David A. Rusling ORCID iD
Author: Keith R. Fox ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×