DNase I footprinting
DNase I footprinting
Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction
978-1-60327-417-3
153-172
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
2009
Cardew, Antonia S.
1a0597fb-0af1-4f42-b21d-a490a04a8dad
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Cardew, Antonia S. and Fox, Keith R.
(2009)
DNase I footprinting.
In,
Drug-DNA Interaction Protocols.
(Methods in Molecular Biology, 613)
New York, US.
Springer, .
(doi:10.1007/978-1-60327-418-0_10).
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Abstract
Footprinting is a method for determining the sequence selectivity of DNA-binding compounds in which ligands protect DNA from cleavage at their binding sites. Footprinting templates are typically 50-200 base pairs long, and DNase I is the most commonly used nuclease for these experiments. This chapter describes the preparation and labelling of suitable DNA footprinting substrates, the footprinting experiment itself, and the way in which these data can be used to estimate the dissociation constant of the interaction
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Published date: 2009
Organisations:
Molecular and Cellular
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Local EPrints ID: 363656
URI: http://eprints.soton.ac.uk/id/eprint/363656
ISBN: 978-1-60327-417-3
PURE UUID: 5158ae16-96d5-4029-a4a2-49eb693f668c
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Date deposited: 28 Mar 2014 16:24
Last modified: 15 Mar 2024 02:36
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Author:
Antonia S. Cardew
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