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A scanning electron microscope characterisation of biofilm on failed craniofacial osteosynthesis miniplates

A scanning electron microscope characterisation of biofilm on failed craniofacial osteosynthesis miniplates
A scanning electron microscope characterisation of biofilm on failed craniofacial osteosynthesis miniplates

INTRODUCTION: Between 3 and 18% of craniofacial osteosynthesis plates are removed due to chronic infection. Removal of the plate is necessary to manage the chronic infective state i.e. miniplate removal results in resolution of the infection. These observations are suggestive of a biofilm-related infection. The aim of this retrospective study was to characterise the presence of biofilm on the removed miniplates from oral and maxillofacial surgery.

MATERIALS AND METHODS: A total of 12 plates and associated screws were recovered from eleven patients suffering from persistent, trauma site infection. The recovered plates plus 1 control plate were imaged using scanning electron microscopy (SEM). One recovered plate was also imaged using confocal microscopy (CM) for comparative purposes.

RESULTS: Of the 12 plates, 3 (25%) demonstrated highly localised polymicrobial biofilms, five (42%) demonstrated coccal biofilms, one possessed a filamentous biofilm and one showed attached yeast. Overall, 75% of the plates and 82% of the patients exhibited evidence of biofilm to varying degrees. All of the infections resolved following removal of the plates and antibiotic treatment.

CONCLUSION: Microbial biofilms can explain the clinical course of chronic infections associated with miniplates.

Bacteria/classification, Biofilms/classification, Bone Plates/microbiology, Bone Screws/microbiology, Cohort Studies, Female, Humans, Male, Mandibular Fractures/surgery, Mandibular Osteotomy/instrumentation, Microscopy, Confocal, Microscopy, Electron, Scanning, Miniaturization, Oral Surgical Procedures/instrumentation, Osteotomy, Le Fort/instrumentation, Prosthesis-Related Infections/pathology, Retrospective Studies, Surface Properties, Surgical Wound Dehiscence/microbiology, Surgical Wound Infection/pathology, Zygomatic Fractures/surgery
1010-5182
e372-e378
Jhass, Aneka K
38124c8f-ecbb-4e20-acdd-cb7aeacfab66
Johnston, David Annandale
b41163c9-b9d2-425c-af99-2a357204014e
Gulati, Aakshay
509ec1e9-3afb-4c02-85d0-70013c014362
Anand, Rajiv
4d4dabe2-5342-4eb1-93c4-20a9762872ff
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Sharma, Sanjay
cbf16e8b-6f5f-499b-8f79-b4a14c0b9e54
Jhass, Aneka K
38124c8f-ecbb-4e20-acdd-cb7aeacfab66
Johnston, David Annandale
b41163c9-b9d2-425c-af99-2a357204014e
Gulati, Aakshay
509ec1e9-3afb-4c02-85d0-70013c014362
Anand, Rajiv
4d4dabe2-5342-4eb1-93c4-20a9762872ff
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Sharma, Sanjay
cbf16e8b-6f5f-499b-8f79-b4a14c0b9e54

Jhass, Aneka K, Johnston, David Annandale, Gulati, Aakshay, Anand, Rajiv, Stoodley, Paul and Sharma, Sanjay (2014) A scanning electron microscope characterisation of biofilm on failed craniofacial osteosynthesis miniplates. Journal of Cranio-Maxillofacial Surgery, 42 (7), e372-e378. (doi:10.1016/j.jcms.2014.03.021).

Record type: Article

Abstract

INTRODUCTION: Between 3 and 18% of craniofacial osteosynthesis plates are removed due to chronic infection. Removal of the plate is necessary to manage the chronic infective state i.e. miniplate removal results in resolution of the infection. These observations are suggestive of a biofilm-related infection. The aim of this retrospective study was to characterise the presence of biofilm on the removed miniplates from oral and maxillofacial surgery.

MATERIALS AND METHODS: A total of 12 plates and associated screws were recovered from eleven patients suffering from persistent, trauma site infection. The recovered plates plus 1 control plate were imaged using scanning electron microscopy (SEM). One recovered plate was also imaged using confocal microscopy (CM) for comparative purposes.

RESULTS: Of the 12 plates, 3 (25%) demonstrated highly localised polymicrobial biofilms, five (42%) demonstrated coccal biofilms, one possessed a filamentous biofilm and one showed attached yeast. Overall, 75% of the plates and 82% of the patients exhibited evidence of biofilm to varying degrees. All of the infections resolved following removal of the plates and antibiotic treatment.

CONCLUSION: Microbial biofilms can explain the clinical course of chronic infections associated with miniplates.

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More information

Published date: 1 April 2014
Additional Information: Copyright © 2014 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
Keywords: Bacteria/classification, Biofilms/classification, Bone Plates/microbiology, Bone Screws/microbiology, Cohort Studies, Female, Humans, Male, Mandibular Fractures/surgery, Mandibular Osteotomy/instrumentation, Microscopy, Confocal, Microscopy, Electron, Scanning, Miniaturization, Oral Surgical Procedures/instrumentation, Osteotomy, Le Fort/instrumentation, Prosthesis-Related Infections/pathology, Retrospective Studies, Surface Properties, Surgical Wound Dehiscence/microbiology, Surgical Wound Infection/pathology, Zygomatic Fractures/surgery
Organisations: nCATS Group

Identifiers

Local EPrints ID: 363744
URI: http://eprints.soton.ac.uk/id/eprint/363744
ISSN: 1010-5182
PURE UUID: 1c7d0cda-8ef9-4ad5-9857-e1c63c768a00
ORCID for David Annandale Johnston: ORCID iD orcid.org/0000-0001-6703-6014
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 03 Apr 2014 08:38
Last modified: 15 Mar 2024 03:34

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Contributors

Author: Aneka K Jhass
Author: David Annandale Johnston ORCID iD
Author: Aakshay Gulati
Author: Rajiv Anand
Author: Paul Stoodley ORCID iD
Author: Sanjay Sharma

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