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Nanotopographical effects on mesenchymal stem cell morphology and phenotype

Nanotopographical effects on mesenchymal stem cell morphology and phenotype
Nanotopographical effects on mesenchymal stem cell morphology and phenotype
There is a rapidly growing body of literature on the effects of topography and critically, nanotopography on cell adhesion, apoptosis and differentiation. Understanding the effects of nanotopography on cell adhesion and morphology and the consequences of cell shape changes in the nucleus, and consequently, gene expression offers new approaches to the elucidation and potential control of stem cell differentiation. In the current study we have used molecular approaches in combination with immunohistology and transcript analysis to understand the role of nanotopography on mesenchymal stem cell morphology and phenotype. Results demonstrate large changes in cell adhesion, nucleus and lamin morphologies in response to the different nanotopographies. Furthermore, these changes relate to alterations in packing of chromosome territories within the interphase nucleus. This, in turn, leads to changes in transcription factor activity and functional (phenotypical) signalling including cell metabolism. Nanotopography provides a useful, non-invasive tool for studying cellular mechanotransduction, gene and protein expression patterns, through effects on cell morphology. The different nanotopographies examined, result in different morphological changes in the cyto- and nucleo-skeleton. We propose that both indirect (biochemical) and direct (mechanical) signalling are important in these early stages of regulating stem cell fate as a consequence of altered metabolic changes and altered phenotype. The current studies provide new insight on cell-surface interactions and enhance our understanding of the modulation of stem cell differentiation with significant potential application in regenerative medicine.
focal adhesions, msc, nanotopography, phenotype
0730-2312
380-390
Tsimbouri, Penelope
bd6fd546-5aba-4fa0-aadc-ab0ccb6b9500
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Burgess, Karl
b1cd8041-8abb-4025-bdba-985cd43bd217
White, Kate
ad592ed1-c133-4e3a-abed-3803a6d5531c
Reynolds, Paul
78fcff19-b4e7-4d37-97d6-680454f20708
Herzyk, Pawel
cef75f09-fa0b-4da4-9050-37df18da040b
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Tsimbouri, Penelope
bd6fd546-5aba-4fa0-aadc-ab0ccb6b9500
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Burgess, Karl
b1cd8041-8abb-4025-bdba-985cd43bd217
White, Kate
ad592ed1-c133-4e3a-abed-3803a6d5531c
Reynolds, Paul
78fcff19-b4e7-4d37-97d6-680454f20708
Herzyk, Pawel
cef75f09-fa0b-4da4-9050-37df18da040b
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc

Tsimbouri, Penelope, Gadegaard, Nikolaj, Burgess, Karl, White, Kate, Reynolds, Paul, Herzyk, Pawel, Oreffo, Richard and Dalby, Matthew J. (2014) Nanotopographical effects on mesenchymal stem cell morphology and phenotype. Journal of Cellular Biochemistry, 115 (2), 380-390. (doi:10.1002/jcb.24673). (PMID:24123223)

Record type: Article

Abstract

There is a rapidly growing body of literature on the effects of topography and critically, nanotopography on cell adhesion, apoptosis and differentiation. Understanding the effects of nanotopography on cell adhesion and morphology and the consequences of cell shape changes in the nucleus, and consequently, gene expression offers new approaches to the elucidation and potential control of stem cell differentiation. In the current study we have used molecular approaches in combination with immunohistology and transcript analysis to understand the role of nanotopography on mesenchymal stem cell morphology and phenotype. Results demonstrate large changes in cell adhesion, nucleus and lamin morphologies in response to the different nanotopographies. Furthermore, these changes relate to alterations in packing of chromosome territories within the interphase nucleus. This, in turn, leads to changes in transcription factor activity and functional (phenotypical) signalling including cell metabolism. Nanotopography provides a useful, non-invasive tool for studying cellular mechanotransduction, gene and protein expression patterns, through effects on cell morphology. The different nanotopographies examined, result in different morphological changes in the cyto- and nucleo-skeleton. We propose that both indirect (biochemical) and direct (mechanical) signalling are important in these early stages of regulating stem cell fate as a consequence of altered metabolic changes and altered phenotype. The current studies provide new insight on cell-surface interactions and enhance our understanding of the modulation of stem cell differentiation with significant potential application in regenerative medicine.

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Published date: February 2014
Keywords: focal adhesions, msc, nanotopography, phenotype
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 367172
URI: http://eprints.soton.ac.uk/id/eprint/367172
ISSN: 0730-2312
PURE UUID: 58909687-3377-43fe-ab32-df96023d861b
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 23 Jul 2014 17:35
Last modified: 15 Mar 2024 03:04

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Contributors

Author: Penelope Tsimbouri
Author: Nikolaj Gadegaard
Author: Karl Burgess
Author: Kate White
Author: Paul Reynolds
Author: Pawel Herzyk
Author: Richard Oreffo ORCID iD
Author: Matthew J. Dalby

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