The University of Southampton
University of Southampton Institutional Repository

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR–ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 106, 1.08±0.11 × 105, 1.03±0.10 × 104, 1.02±0.09 × 103, 1.04±0.10 × 102 and 10.0±1.5 copies/?l. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR–ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR–ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa.eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f).
0887-6924
369-376
White, H.
2181c0b9-fc3b-407e-95eb-3510524603e5
Deprez, L.
437e7cb5-adc2-4e9e-b634-291233c44ba7
Corbisier, P.
6e6fa432-2662-49f2-85ee-79a2b5d57152
Hall, V.
392c6a79-0e75-4a0b-87b4-61de4d1466c9
Lin, F.
9067ee54-a57f-4432-b390-ec81fd8aa73c
Mazoua, S.
21e13b1b-eebd-440e-b2f6-84965ec6331f
Trapmann, S.
de71ce4f-0bb1-40bb-9e3e-512efcdc86ef
Aggerholm, A.
f438ce0d-0e60-4238-971d-5dfec8512674
Andrikovics, H.
14e9191e-2a63-4518-bae2-2d24a4cac77e
Akiki, S.
d6896048-e77f-4773-9fc5-a42e9ea5a1cc
Barbany, G.
6e0a02fb-f663-4741-862c-34d78bc74bfc
Boeckx, N.
075d6c2f-b301-44e0-a5cb-6414f40feee6
Bench, A.
8f081ec8-ed7b-4017-bfac-de87749575f5
Catherwood, M.
6d0b4df0-ad48-465e-8cf5-d292f93c114f
Cayuela, J-M.
8bb662e8-31bc-4553-ae73-30bb33820f5d
Chudleigh, S.
51b6015a-888f-47f4-96cb-08f72f6ce4c7
Clench, T.
9a317eb3-3a31-46c7-9e32-9ee46510b5b3
Colomer, D.
fce349a2-3c24-4173-8095-6aca7903719a
Daraio, F.
c3c8805a-962d-44eb-8b4e-02d615049265
Dulucq, S.
031885b7-1462-4202-95c8-539eb9b03224
Farrugia, J.
8566f038-170c-4f98-92f7-32af1c80eaec
Fletcher, L.
aa33d255-f749-438f-abaf-2e5a26f33221
Foroni, L.
dd5521ed-04aa-4049-9624-7eaef86dec43
Ganderton, R.
6c4ffe96-7eb0-4e77-9c17-306887497187
Gerrard, G.
1d1a5195-6354-44bd-874d-06ca6b07f589
Gineikienė, E.
64aa20f9-b979-47a4-a6e1-36579d2823db
Hayette, S.
c652c50a-c69d-4960-9be1-1976ce6065e5
El Housni, H.
5afb3ac2-ae97-46ba-b1e9-3ec047b8c6bd
Izzo, B.
e547cb70-1083-420c-baf4-be89a0c7ff3a
Jansson, M.
58e7a397-0b88-4386-a72b-c5a11b3a0054
Johnels, P.
7f747e2f-927e-4dee-b0d3-3f90aa25ae8c
Jurcek, T.
8945a5ec-f6d1-423b-ab9a-acdd7bc73e84
Kairisto, V.
7b3d1fff-53af-49ae-9fc7-ba35a1757fe7
Kizilors, A.
9ce74ae5-c195-412a-82de-d4febb1dd374
Kim, D-W.
06a9e732-bd09-4e48-95e7-0d24a1b5956c
Lange, T.
52ec27c6-1863-405e-be80-7c65d7bd03df
Lion, T.
05bb213d-5f98-4a16-b49e-d7077ca3287d
Polakova, K.M.
5714785d-ed69-4e2f-9a79-edd61842688d
Martinelli, G.
3949da7a-7efe-4ebd-b1f7-92f1ca150d66
McCarron, S.
1a75c9be-54b3-48e5-a21d-9fda1d5763c3
Merle, P.A.
0140ddcb-cc9a-42b1-bdea-6bfe70760142
Milner, B.
8d5eb9bc-4c09-4915-bab5-2dacc7a18042
Mitterbauer-Hohendanner, G.
46cea1ef-f8bb-43ff-88dc-cd1a60ff11e2
Nagar, M.
cc8b4df4-54a4-40ec-998a-40382836ac52
Nickless, G.
60071889-e53f-4372-b19e-c09598e0b1a0
Nomdedéu, J.
fac48e0e-910e-442c-ab7b-1ffd45712718
Nymoen, D.A.
3a40dd49-6c63-4954-9e52-42e19eec2e23
Leibundgut, E.O.
13113bd7-5064-4190-a0e9-2503bccb310c
Ozbek, U.
db9deb94-26bc-42cd-98c9-60d89deb71ea
Pajič, T.
5d0ea973-7f9b-422d-8afd-96cf7476507a
Pfeifer, H.
f31aea3b-8ae3-458b-8cef-8022de7ed998
Preudhomme, C.
1db28c45-3add-43f1-96b7-09d2c8442c66
Raudsepp, K.
2cd09b92-9e1d-40cc-a971-9643840cae1a
Romeo, G.
5376216a-3678-44ca-83d8-f1d03ddae3dc
Sacha, T.
194f2d6b-2d75-417a-9bda-dfa1881170f2
Talmaci, R.
3777adf3-77a9-4a40-afb2-7002c7ad3232
Touloumenidou, T.
0f7c4d69-6fd5-493f-8f7d-1c49b7e38fa2
Van der Velden, V.H.J.
f98acfa2-a86d-4367-82b4-512d0f091bf8
Waits, P.
96176396-406b-4514-9dff-88664beeeec1
Wang, L.
ff28fc9d-dbeb-460f-943c-f49997f58bf7
Wilkinson, E.
eb586979-85f1-4286-87e7-91c73f5f55c1
Wilson, G.
8fd34f42-b70e-48a6-b256-cd6f938aa8c1
Wren, D.
8c294e7e-000e-4345-afb2-bb7063bc5371
Zadro, R.
dfe18af1-1f06-43e6-8101-0ef54d6e9e9d
Ziermann, J.
98a410c6-7328-4daa-9673-90920b8a9b9e
Zoi, K.
a9f569b2-4539-4efd-8ac8-c21f0d347225
Müller, M.C.
822204b1-eb93-40ae-a110-6cc912503bcd
Hochhaus, A.
4c0b9da4-adfa-4253-8af7-78cec9e9a24f
Schimmel, H.
27bbf9d8-bbac-4ac8-a302-ac37f77fc5dc
Cross, N. C. P.
f87650da-b908-4a34-b31b-d62c5f186fe4
Emons, H.
3ffecae4-8b41-41a0-9a14-27ace726521c
White, H.
2181c0b9-fc3b-407e-95eb-3510524603e5
Deprez, L.
437e7cb5-adc2-4e9e-b634-291233c44ba7
Corbisier, P.
6e6fa432-2662-49f2-85ee-79a2b5d57152
Hall, V.
392c6a79-0e75-4a0b-87b4-61de4d1466c9
Lin, F.
9067ee54-a57f-4432-b390-ec81fd8aa73c
Mazoua, S.
21e13b1b-eebd-440e-b2f6-84965ec6331f
Trapmann, S.
de71ce4f-0bb1-40bb-9e3e-512efcdc86ef
Aggerholm, A.
f438ce0d-0e60-4238-971d-5dfec8512674
Andrikovics, H.
14e9191e-2a63-4518-bae2-2d24a4cac77e
Akiki, S.
d6896048-e77f-4773-9fc5-a42e9ea5a1cc
Barbany, G.
6e0a02fb-f663-4741-862c-34d78bc74bfc
Boeckx, N.
075d6c2f-b301-44e0-a5cb-6414f40feee6
Bench, A.
8f081ec8-ed7b-4017-bfac-de87749575f5
Catherwood, M.
6d0b4df0-ad48-465e-8cf5-d292f93c114f
Cayuela, J-M.
8bb662e8-31bc-4553-ae73-30bb33820f5d
Chudleigh, S.
51b6015a-888f-47f4-96cb-08f72f6ce4c7
Clench, T.
9a317eb3-3a31-46c7-9e32-9ee46510b5b3
Colomer, D.
fce349a2-3c24-4173-8095-6aca7903719a
Daraio, F.
c3c8805a-962d-44eb-8b4e-02d615049265
Dulucq, S.
031885b7-1462-4202-95c8-539eb9b03224
Farrugia, J.
8566f038-170c-4f98-92f7-32af1c80eaec
Fletcher, L.
aa33d255-f749-438f-abaf-2e5a26f33221
Foroni, L.
dd5521ed-04aa-4049-9624-7eaef86dec43
Ganderton, R.
6c4ffe96-7eb0-4e77-9c17-306887497187
Gerrard, G.
1d1a5195-6354-44bd-874d-06ca6b07f589
Gineikienė, E.
64aa20f9-b979-47a4-a6e1-36579d2823db
Hayette, S.
c652c50a-c69d-4960-9be1-1976ce6065e5
El Housni, H.
5afb3ac2-ae97-46ba-b1e9-3ec047b8c6bd
Izzo, B.
e547cb70-1083-420c-baf4-be89a0c7ff3a
Jansson, M.
58e7a397-0b88-4386-a72b-c5a11b3a0054
Johnels, P.
7f747e2f-927e-4dee-b0d3-3f90aa25ae8c
Jurcek, T.
8945a5ec-f6d1-423b-ab9a-acdd7bc73e84
Kairisto, V.
7b3d1fff-53af-49ae-9fc7-ba35a1757fe7
Kizilors, A.
9ce74ae5-c195-412a-82de-d4febb1dd374
Kim, D-W.
06a9e732-bd09-4e48-95e7-0d24a1b5956c
Lange, T.
52ec27c6-1863-405e-be80-7c65d7bd03df
Lion, T.
05bb213d-5f98-4a16-b49e-d7077ca3287d
Polakova, K.M.
5714785d-ed69-4e2f-9a79-edd61842688d
Martinelli, G.
3949da7a-7efe-4ebd-b1f7-92f1ca150d66
McCarron, S.
1a75c9be-54b3-48e5-a21d-9fda1d5763c3
Merle, P.A.
0140ddcb-cc9a-42b1-bdea-6bfe70760142
Milner, B.
8d5eb9bc-4c09-4915-bab5-2dacc7a18042
Mitterbauer-Hohendanner, G.
46cea1ef-f8bb-43ff-88dc-cd1a60ff11e2
Nagar, M.
cc8b4df4-54a4-40ec-998a-40382836ac52
Nickless, G.
60071889-e53f-4372-b19e-c09598e0b1a0
Nomdedéu, J.
fac48e0e-910e-442c-ab7b-1ffd45712718
Nymoen, D.A.
3a40dd49-6c63-4954-9e52-42e19eec2e23
Leibundgut, E.O.
13113bd7-5064-4190-a0e9-2503bccb310c
Ozbek, U.
db9deb94-26bc-42cd-98c9-60d89deb71ea
Pajič, T.
5d0ea973-7f9b-422d-8afd-96cf7476507a
Pfeifer, H.
f31aea3b-8ae3-458b-8cef-8022de7ed998
Preudhomme, C.
1db28c45-3add-43f1-96b7-09d2c8442c66
Raudsepp, K.
2cd09b92-9e1d-40cc-a971-9643840cae1a
Romeo, G.
5376216a-3678-44ca-83d8-f1d03ddae3dc
Sacha, T.
194f2d6b-2d75-417a-9bda-dfa1881170f2
Talmaci, R.
3777adf3-77a9-4a40-afb2-7002c7ad3232
Touloumenidou, T.
0f7c4d69-6fd5-493f-8f7d-1c49b7e38fa2
Van der Velden, V.H.J.
f98acfa2-a86d-4367-82b4-512d0f091bf8
Waits, P.
96176396-406b-4514-9dff-88664beeeec1
Wang, L.
ff28fc9d-dbeb-460f-943c-f49997f58bf7
Wilkinson, E.
eb586979-85f1-4286-87e7-91c73f5f55c1
Wilson, G.
8fd34f42-b70e-48a6-b256-cd6f938aa8c1
Wren, D.
8c294e7e-000e-4345-afb2-bb7063bc5371
Zadro, R.
dfe18af1-1f06-43e6-8101-0ef54d6e9e9d
Ziermann, J.
98a410c6-7328-4daa-9673-90920b8a9b9e
Zoi, K.
a9f569b2-4539-4efd-8ac8-c21f0d347225
Müller, M.C.
822204b1-eb93-40ae-a110-6cc912503bcd
Hochhaus, A.
4c0b9da4-adfa-4253-8af7-78cec9e9a24f
Schimmel, H.
27bbf9d8-bbac-4ac8-a302-ac37f77fc5dc
Cross, N. C. P.
f87650da-b908-4a34-b31b-d62c5f186fe4
Emons, H.
3ffecae4-8b41-41a0-9a14-27ace726521c

White, H., Deprez, L., Corbisier, P., Hall, V., Lin, F., Mazoua, S., Trapmann, S., Aggerholm, A., Andrikovics, H., Akiki, S., Barbany, G., Boeckx, N., Bench, A., Catherwood, M., Cayuela, J-M., Chudleigh, S., Clench, T., Colomer, D., Daraio, F., Dulucq, S., Farrugia, J., Fletcher, L., Foroni, L., Ganderton, R., Gerrard, G., Gineikienė, E., Hayette, S., El Housni, H., Izzo, B., Jansson, M., Johnels, P., Jurcek, T., Kairisto, V., Kizilors, A., Kim, D-W., Lange, T., Lion, T., Polakova, K.M., Martinelli, G., McCarron, S., Merle, P.A., Milner, B., Mitterbauer-Hohendanner, G., Nagar, M., Nickless, G., Nomdedéu, J., Nymoen, D.A., Leibundgut, E.O., Ozbek, U., Pajič, T., Pfeifer, H., Preudhomme, C., Raudsepp, K., Romeo, G., Sacha, T., Talmaci, R., Touloumenidou, T., Van der Velden, V.H.J., Waits, P., Wang, L., Wilkinson, E., Wilson, G., Wren, D., Zadro, R., Ziermann, J., Zoi, K., Müller, M.C., Hochhaus, A., Schimmel, H., Cross, N. C. P. and Emons, H. (2015) A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR. Leukemia, 29 (2), 369-376. (doi:10.1038/leu.2014.217). (PMID:25036192)

Record type: Article

Abstract

Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR–ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 106, 1.08±0.11 × 105, 1.03±0.10 × 104, 1.02±0.09 × 103, 1.04±0.10 × 102 and 10.0±1.5 copies/?l. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR–ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR–ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa.eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f).

Text
__userfiles.soton.ac.uk_Users_slb1_mydocuments_leu2014217a.pdf - Other
Available under License Other.
Download (676kB)

More information

e-pub ahead of print date: 12 August 2014
Published date: February 2015
Organisations: Faculty of Medicine, Human Development & Health

Identifiers

Local EPrints ID: 367226
URI: http://eprints.soton.ac.uk/id/eprint/367226
ISSN: 0887-6924
PURE UUID: 4f8c3a83-9291-47c2-bdd8-42a29be73712
ORCID for N. C. P. Cross: ORCID iD orcid.org/0000-0001-5481-2555

Catalogue record

Date deposited: 19 Aug 2014 09:58
Last modified: 15 Mar 2024 03:11

Export record

Altmetrics

Contributors

Author: H. White
Author: L. Deprez
Author: P. Corbisier
Author: V. Hall
Author: F. Lin
Author: S. Mazoua
Author: S. Trapmann
Author: A. Aggerholm
Author: H. Andrikovics
Author: S. Akiki
Author: G. Barbany
Author: N. Boeckx
Author: A. Bench
Author: M. Catherwood
Author: J-M. Cayuela
Author: S. Chudleigh
Author: T. Clench
Author: D. Colomer
Author: F. Daraio
Author: S. Dulucq
Author: J. Farrugia
Author: L. Fletcher
Author: L. Foroni
Author: R. Ganderton
Author: G. Gerrard
Author: E. Gineikienė
Author: S. Hayette
Author: H. El Housni
Author: B. Izzo
Author: M. Jansson
Author: P. Johnels
Author: T. Jurcek
Author: V. Kairisto
Author: A. Kizilors
Author: D-W. Kim
Author: T. Lange
Author: T. Lion
Author: K.M. Polakova
Author: G. Martinelli
Author: S. McCarron
Author: P.A. Merle
Author: B. Milner
Author: G. Mitterbauer-Hohendanner
Author: M. Nagar
Author: G. Nickless
Author: J. Nomdedéu
Author: D.A. Nymoen
Author: E.O. Leibundgut
Author: U. Ozbek
Author: T. Pajič
Author: H. Pfeifer
Author: C. Preudhomme
Author: K. Raudsepp
Author: G. Romeo
Author: T. Sacha
Author: R. Talmaci
Author: T. Touloumenidou
Author: V.H.J. Van der Velden
Author: P. Waits
Author: L. Wang
Author: E. Wilkinson
Author: G. Wilson
Author: D. Wren
Author: R. Zadro
Author: J. Ziermann
Author: K. Zoi
Author: M.C. Müller
Author: A. Hochhaus
Author: H. Schimmel
Author: N. C. P. Cross ORCID iD
Author: H. Emons

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×