Design and validation of an in vitro loading system for the combined application of cyclic compression and shear to 3D chondrocytes-seeded agarose constructs
Design and validation of an in vitro loading system for the combined application of cyclic compression and shear to 3D chondrocytes-seeded agarose constructs
Physiological loading is essential for the maintenance of articular cartilage by regulating tissue remodelling, in the form of both catabolic and anabolic processes. To promote the development of tissue engineered cartilage which closely matches the long term functionality of native tissue, bioreactors have been developed to provide a combination of loading modalities, which reflect the nature of normal physiological loads. This study describes the design and validation of an in vitro mechanical system for the controlled application of bi-axial loading regimes to chondrocyte-seeded agarose constructs.
The computer-controlled system incorporates a robust gripping system, which ensures the delivery of precise values of cyclic compressive and shear strain to 3D cell-seeded constructs. Sample prototypes were designed, optimised using finite element analysis and validated performing compressive and shear fatigue mechanical tests. The horizontal and vertical displacements within the bioreactor are precisely controlled by a dedicated programme that can be easily implemented. The synchronisation of the orthogonal displacements was shown to be accurate and reproducible.
Constructs were successfully loaded with a combined compressive and shear loading regimen at 1 Hz for up to 48 h with no appreciable loss of cell viability or mechanical integrity. These features along with the demonstrated high consistency make the system ideally suitable for a systematic investigation of the response of chondrocytes to a complex physiologically relevant deformation profile.
534-540
Di Federico, Erica
66063cda-3d6c-4465-bedc-c63ea3d2ff56
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
April 2014
Di Federico, Erica
66063cda-3d6c-4465-bedc-c63ea3d2ff56
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Shelton, Julia C.
04ee5976-0824-48a8-ae1d-b0e147d8a879
Di Federico, Erica, Bader, Dan L. and Shelton, Julia C.
(2014)
Design and validation of an in vitro loading system for the combined application of cyclic compression and shear to 3D chondrocytes-seeded agarose constructs.
Medical Engineering & Physics, 36 (4), .
(doi:10.1016/j.medengphy.2013.11.007).
(PMID:24355317)
Abstract
Physiological loading is essential for the maintenance of articular cartilage by regulating tissue remodelling, in the form of both catabolic and anabolic processes. To promote the development of tissue engineered cartilage which closely matches the long term functionality of native tissue, bioreactors have been developed to provide a combination of loading modalities, which reflect the nature of normal physiological loads. This study describes the design and validation of an in vitro mechanical system for the controlled application of bi-axial loading regimes to chondrocyte-seeded agarose constructs.
The computer-controlled system incorporates a robust gripping system, which ensures the delivery of precise values of cyclic compressive and shear strain to 3D cell-seeded constructs. Sample prototypes were designed, optimised using finite element analysis and validated performing compressive and shear fatigue mechanical tests. The horizontal and vertical displacements within the bioreactor are precisely controlled by a dedicated programme that can be easily implemented. The synchronisation of the orthogonal displacements was shown to be accurate and reproducible.
Constructs were successfully loaded with a combined compressive and shear loading regimen at 1 Hz for up to 48 h with no appreciable loss of cell viability or mechanical integrity. These features along with the demonstrated high consistency make the system ideally suitable for a systematic investigation of the response of chondrocytes to a complex physiologically relevant deformation profile.
This record has no associated files available for download.
More information
e-pub ahead of print date: 17 December 2013
Published date: April 2014
Organisations:
Faculty of Health Sciences
Identifiers
Local EPrints ID: 369165
URI: http://eprints.soton.ac.uk/id/eprint/369165
ISSN: 1350-4533
PURE UUID: acfd2420-0e20-4dc9-b612-81842e45b117
Catalogue record
Date deposited: 29 Sep 2014 10:25
Last modified: 14 Mar 2024 17:59
Export record
Altmetrics
Contributors
Author:
Erica Di Federico
Author:
Julia C. Shelton
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
