Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin
Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin
Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-?1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.
TSGA10, vimentin, dendritic cell, macrophage, intermediate filament
120-126
Roghanian, Ali
e2b032c2-60a0-4522-a3d8-56a768792f36
Jones, Des C.
f82edca9-6a9a-43c6-9378-7d435c833bc5
Pattisapu, Jogi V.
08b7aaec-c23e-4f5c-9761-f9643acff02c
Wolfe, Jonathan
3dc6b748-9241-4c57-8f90-182cc2f44a51
Young, Neil T.
420c5bc3-494e-4840-9af2-dc1b081291b1
Behnam, Babak
bb3a7da7-42d7-40c0-ac86-18bdecb71732
2010
Roghanian, Ali
e2b032c2-60a0-4522-a3d8-56a768792f36
Jones, Des C.
f82edca9-6a9a-43c6-9378-7d435c833bc5
Pattisapu, Jogi V.
08b7aaec-c23e-4f5c-9761-f9643acff02c
Wolfe, Jonathan
3dc6b748-9241-4c57-8f90-182cc2f44a51
Young, Neil T.
420c5bc3-494e-4840-9af2-dc1b081291b1
Behnam, Babak
bb3a7da7-42d7-40c0-ac86-18bdecb71732
Roghanian, Ali, Jones, Des C., Pattisapu, Jogi V., Wolfe, Jonathan, Young, Neil T. and Behnam, Babak
(2010)
Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin.
Cellular Immunology, 265 (2), .
(doi:10.1016/j.cellimm.2010.07.012).
(PMID:20797700)
Abstract
Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-?1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.
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e-pub ahead of print date: 2 August 2010
Published date: 2010
Keywords:
TSGA10, vimentin, dendritic cell, macrophage, intermediate filament
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 369609
URI: http://eprints.soton.ac.uk/id/eprint/369609
ISSN: 0008-8749
PURE UUID: 9589ccbb-3f44-48eb-88ff-f521e569b175
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Date deposited: 02 Oct 2014 10:39
Last modified: 15 Mar 2024 03:34
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Contributors
Author:
Des C. Jones
Author:
Jogi V. Pattisapu
Author:
Jonathan Wolfe
Author:
Neil T. Young
Author:
Babak Behnam
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