A Structure-Activity study of the neuropeptide PF1, SDPNFLRFamide, using the dorsal body wall muscle of the chicken nematode, Ascaridia galli
A Structure-Activity study of the neuropeptide PF1, SDPNFLRFamide, using the dorsal body wall muscle of the chicken nematode, Ascaridia galli
The action of a range of N terminally modified peptides structurally related to the nematode peptide PF1, SDPNFLRFamide, has been investigated using a dorsal muscle strip preparation from the chicken nematode, Ascaridia galli. Acetylcholine contracts this muscle preparation in a concentration-dependent manner when applied in the range 1-100 µM with an EC50 value of 9 µM. These contractions are reduced in the presence of PF1 and its analogues, with a threshold effect of PF1 of around 1 nM and an IC50 value of 470 nM against 10 µM acetylcholine. All the PF1 analogues tested were less potent than PF1 in reducing the acetylcholine contractions, indicating the importance of the N terminal amino acids in the action of PF1 in this preparation
ascaridia galli, pf1, sdpnflrfamide, acetylcholine, dorsal muscle strip
343-351
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Franks, C.J.
9842534b-4d3f-4ee8-a07e-3b050f748593
May 2004
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Franks, C.J.
9842534b-4d3f-4ee8-a07e-3b050f748593
Holden-Dye, Lindy, Walker, R.J. and Franks, C.J.
(2004)
A Structure-Activity study of the neuropeptide PF1, SDPNFLRFamide, using the dorsal body wall muscle of the chicken nematode, Ascaridia galli.
Acta Biologica Hungarica, 55 (1), .
(doi:10.1556/ABiol.55.2004.1-4.40).
(PMID:15270251)
Abstract
The action of a range of N terminally modified peptides structurally related to the nematode peptide PF1, SDPNFLRFamide, has been investigated using a dorsal muscle strip preparation from the chicken nematode, Ascaridia galli. Acetylcholine contracts this muscle preparation in a concentration-dependent manner when applied in the range 1-100 µM with an EC50 value of 9 µM. These contractions are reduced in the presence of PF1 and its analogues, with a threshold effect of PF1 of around 1 nM and an IC50 value of 470 nM against 10 µM acetylcholine. All the PF1 analogues tested were less potent than PF1 in reducing the acetylcholine contractions, indicating the importance of the N terminal amino acids in the action of PF1 in this preparation
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Published date: May 2004
Keywords:
ascaridia galli, pf1, sdpnflrfamide, acetylcholine, dorsal muscle strip
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 372590
URI: http://eprints.soton.ac.uk/id/eprint/372590
ISSN: 0236-5383
PURE UUID: 926f3b34-e730-43ae-b21a-f518f62e4467
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Date deposited: 09 Dec 2014 13:31
Last modified: 15 Mar 2024 02:52
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Author:
R.J. Walker
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