ABC50 mutants modify translation start codon selection
ABC50 mutants modify translation start codon selection
ABC50 (also known as ABCF1) binds to eukaryotic initiation factor eIF2 and is required for efficient translation initiation. An essential step of this process is accurate recognition and selection of the initiation codon. It is widely accepted that the presence and movement of eIF1, eIF1A and eIF5 are key factors in modulating the stringency of start site selection, which normally requires an AUG in an appropriate sequence context. Here we show that expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. These mutants do not appear to alter the association of key initiation factors to 40S subunits. The stringency of start site selection can be restored through overexpression of eIF1, consistent with the role of that factor in enhancing stringency. This study indicates that interfering with the function of ABC50 influences the accuracy of initiation codon selection
217-229
Stewart, Joanna D
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Cowan, Joanne L.
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Perry, Lisa S.
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Coldwell, Mark J.
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Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3
Stewart, Joanna D
e1ec9784-39cc-48ed-9f4f-2a05d25f2106
Cowan, Joanne L.
a34fc26a-e7a3-435f-85c8-4f196b1c8d63
Perry, Lisa S.
54503067-f509-4deb-9c54-6f656787894b
Coldwell, Mark J.
a3432799-ed45-4948-9f7a-2a284d3ec65c
Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3
Stewart, Joanna D, Cowan, Joanne L., Perry, Lisa S., Coldwell, Mark J. and Proud, Christopher G.
(2015)
ABC50 mutants modify translation start codon selection.
Biochemical Journal, 467 (2), .
(doi:10.1042/BJ20141453).
(PMID:25597744)
Abstract
ABC50 (also known as ABCF1) binds to eukaryotic initiation factor eIF2 and is required for efficient translation initiation. An essential step of this process is accurate recognition and selection of the initiation codon. It is widely accepted that the presence and movement of eIF1, eIF1A and eIF5 are key factors in modulating the stringency of start site selection, which normally requires an AUG in an appropriate sequence context. Here we show that expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. These mutants do not appear to alter the association of key initiation factors to 40S subunits. The stringency of start site selection can be restored through overexpression of eIF1, consistent with the role of that factor in enhancing stringency. This study indicates that interfering with the function of ABC50 influences the accuracy of initiation codon selection
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Accepted/In Press date: 19 January 2015
e-pub ahead of print date: 19 January 2015
Organisations:
Molecular and Cellular
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Local EPrints ID: 373653
URI: http://eprints.soton.ac.uk/id/eprint/373653
ISSN: 1470-8728
PURE UUID: dcc70286-9baf-430c-bf7c-d4860a305c68
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Date deposited: 26 Jan 2015 13:28
Last modified: 14 Mar 2024 18:55
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Author:
Joanna D Stewart
Author:
Lisa S. Perry
Author:
Mark J. Coldwell
Author:
Christopher G. Proud
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