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Bridging two cultures: minimalistic networks prepared by microfluidic arraying, and open access compartments for electrophysiology

Bridging two cultures: minimalistic networks prepared by microfluidic arraying, and open access compartments for electrophysiology
Bridging two cultures: minimalistic networks prepared by microfluidic arraying, and open access compartments for electrophysiology
Microfabrication protocols are described for two compartmentalized neuron culture platforms which extend beyond the capabilities of conventional systems. The first involves a differential flow microfluidic circuit for arraying single neurons, along with protocols for in chip biomaterial patterning and the selective treatment of somata or outgrowth compartments. These minimalistic neuronal networks are ideal for spatially resolved research using rare and precious neuronal subtypes as well as parallelization for screening biochemical libraries. The second, open-access, system solves the micro-to-macro interface challenge to enable the insertion of micromanipulators for electrophysiology studies or localized perturbation using a microinjector. This system is especially useful for the spatiotemporal investigation of mechanisms underlying disease, such as neurodegeneration and epileptic seizures. Design files along with soft lithography replication techniques are provided to facilitate the straightforward uptake of these platforms. Technology integration approaches are also presented for the alignment of arrayed neurons with individual microelectrodes for highly parallel electrophysiological and electrochemical measurements throughout nodes in the compartmentalized neuronal network.
soft lithography, user-friendly, cell patterning, microfluidics, electrophysiology, neurodegeneration, alzheimer and Parkinson disease, epilepsy, long-range signaling, blood–brain barrier
978-1-4939-2509-4
0893-2336
103
39-56
Humana Press
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Dinh, Ngoc-Duy
caa5d6c7-79aa-47c4-9612-a54898102fb8
Hardelauf, Heike
ff18e0f8-62ed-4edf-9746-f72701657440
Chiang, Ya-Yu
0ab4fe9e-9a3e-4594-a13c-73f68c13b8b3
Newman, Tracey
322290cb-2e9c-445d-a047-00b1bea39a25
Vargas-Caballero, Mariana
de2178ac-77fd-4748-9fe5-109ab8ad93e1
Asuni, Ayodeji A.
ff307ab4-a7ae-4bcf-be87-86923ebcb0c7
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Biffi, Emilia
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Dinh, Ngoc-Duy
caa5d6c7-79aa-47c4-9612-a54898102fb8
Hardelauf, Heike
ff18e0f8-62ed-4edf-9746-f72701657440
Chiang, Ya-Yu
0ab4fe9e-9a3e-4594-a13c-73f68c13b8b3
Newman, Tracey
322290cb-2e9c-445d-a047-00b1bea39a25
Vargas-Caballero, Mariana
de2178ac-77fd-4748-9fe5-109ab8ad93e1
Asuni, Ayodeji A.
ff307ab4-a7ae-4bcf-be87-86923ebcb0c7
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Biffi, Emilia

West, Jonathan, Dinh, Ngoc-Duy, Hardelauf, Heike, Chiang, Ya-Yu, Newman, Tracey, Vargas-Caballero, Mariana, Asuni, Ayodeji A., Deinhardt, Katrin and Arundell, Martin (2015) Bridging two cultures: minimalistic networks prepared by microfluidic arraying, and open access compartments for electrophysiology. In, Biffi, Emilia (ed.) Microfluidic and Compartmentalized Platforms for Neurobiological Research. (Neuromethods, 103) New York, US. Humana Press, pp. 39-56. (doi:10.1007/978-1-4939-2510-0_3).

Record type: Book Section

Abstract

Microfabrication protocols are described for two compartmentalized neuron culture platforms which extend beyond the capabilities of conventional systems. The first involves a differential flow microfluidic circuit for arraying single neurons, along with protocols for in chip biomaterial patterning and the selective treatment of somata or outgrowth compartments. These minimalistic neuronal networks are ideal for spatially resolved research using rare and precious neuronal subtypes as well as parallelization for screening biochemical libraries. The second, open-access, system solves the micro-to-macro interface challenge to enable the insertion of micromanipulators for electrophysiology studies or localized perturbation using a microinjector. This system is especially useful for the spatiotemporal investigation of mechanisms underlying disease, such as neurodegeneration and epileptic seizures. Design files along with soft lithography replication techniques are provided to facilitate the straightforward uptake of these platforms. Technology integration approaches are also presented for the alignment of arrayed neurons with individual microelectrodes for highly parallel electrophysiological and electrochemical measurements throughout nodes in the compartmentalized neuronal network.

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Published date: 19 February 2015
Keywords: soft lithography, user-friendly, cell patterning, microfluidics, electrophysiology, neurodegeneration, alzheimer and Parkinson disease, epilepsy, long-range signaling, blood–brain barrier
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 375885
URI: http://eprints.soton.ac.uk/id/eprint/375885
ISBN: 978-1-4939-2509-4
ISSN: 0893-2336
PURE UUID: 721b5b58-d3fe-48e4-a2e2-81dd0a9acfe7
ORCID for Jonathan West: ORCID iD orcid.org/0000-0002-5709-6790
ORCID for Tracey Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for Mariana Vargas-Caballero: ORCID iD orcid.org/0000-0003-2326-4001
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298

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Date deposited: 22 Apr 2015 12:00
Last modified: 15 Mar 2024 03:45

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Contributors

Author: Jonathan West ORCID iD
Author: Ngoc-Duy Dinh
Author: Heike Hardelauf
Author: Ya-Yu Chiang
Author: Tracey Newman ORCID iD
Author: Ayodeji A. Asuni
Author: Martin Arundell
Editor: Emilia Biffi

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