Dietary docosahexaenoic acid and eicosapentaenoic acid influence liver triacylglycerol and insulin resistance in rats fed a high-fructose diet
Dietary docosahexaenoic acid and eicosapentaenoic acid influence liver triacylglycerol and insulin resistance in rats fed a high-fructose diet
This study aimed to examine the benefits of different amounts of omega-3 (n-3) polyunsaturated fatty acids from fish oil (FO) on lipid metabolism, insulin resistance and gene expression in rats fed a high-fructose diet. Male Wistar rats were separated into two groups: Control (C, n = 6) and Fructose (Fr, n = 32), the latter receiving a diet containing 63% by weight fructose for 60 days. After this period, 24 animals from Fr group were allocated to three groups: FrFO2 (n = 8) receiving 63% fructose and 2% FO plus 5% soybean oil; FrFO5 (n = 8) receiving 63% fructose and 5% FO plus 2% soybean oil; and FrFO7 (n = 8) receiving 63% fructose and 7% FO. Animals were fed these diets for 30 days. Fructose led to an increase in liver weight, hepatic and serum triacylglycerol, serum alanine aminotransferase and HOMA1-IR index. These alterations were reversed by 5% and 7% FO. FO had a dose-dependent effect on expression of genes related to hepatic ?-oxidation (increased) and hepatic lipogenesis (decreased). The group receiving the highest FO amount had increased markers of oxidative stress. It is concluded that n-3 fatty acids may be able to reverse the adverse metabolic effects induced by a high fructose diet
fish oil, fructose, metabolic syndrome, omega-3 fatty acids
1864-1881
de Castro, G.S.
33a6f832-af11-4b0a-8dd6-9b69e66b6434
Deminice, R.
de0fd150-007b-4dfc-a615-7154895d6c0a
Simoes-Ambrosio, L.M.C.
f1aebfe8-cf51-4c1d-a8c7-149cac3c4b8d
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jordao, A.A.
9b88657b-6f3e-43a4-b168-37ada40f185b
Vannucchi, H.
9b8ff52d-feb3-4d65-9bc8-4d829c8b880c
1 April 2015
de Castro, G.S.
33a6f832-af11-4b0a-8dd6-9b69e66b6434
Deminice, R.
de0fd150-007b-4dfc-a615-7154895d6c0a
Simoes-Ambrosio, L.M.C.
f1aebfe8-cf51-4c1d-a8c7-149cac3c4b8d
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jordao, A.A.
9b88657b-6f3e-43a4-b168-37ada40f185b
Vannucchi, H.
9b8ff52d-feb3-4d65-9bc8-4d829c8b880c
de Castro, G.S., Deminice, R., Simoes-Ambrosio, L.M.C., Calder, P.C., Jordao, A.A. and Vannucchi, H.
(2015)
Dietary docosahexaenoic acid and eicosapentaenoic acid influence liver triacylglycerol and insulin resistance in rats fed a high-fructose diet.
Marine Drugs, 13, .
(doi:10.3390/md13041864).
(PMID:25837985)
Abstract
This study aimed to examine the benefits of different amounts of omega-3 (n-3) polyunsaturated fatty acids from fish oil (FO) on lipid metabolism, insulin resistance and gene expression in rats fed a high-fructose diet. Male Wistar rats were separated into two groups: Control (C, n = 6) and Fructose (Fr, n = 32), the latter receiving a diet containing 63% by weight fructose for 60 days. After this period, 24 animals from Fr group were allocated to three groups: FrFO2 (n = 8) receiving 63% fructose and 2% FO plus 5% soybean oil; FrFO5 (n = 8) receiving 63% fructose and 5% FO plus 2% soybean oil; and FrFO7 (n = 8) receiving 63% fructose and 7% FO. Animals were fed these diets for 30 days. Fructose led to an increase in liver weight, hepatic and serum triacylglycerol, serum alanine aminotransferase and HOMA1-IR index. These alterations were reversed by 5% and 7% FO. FO had a dose-dependent effect on expression of genes related to hepatic ?-oxidation (increased) and hepatic lipogenesis (decreased). The group receiving the highest FO amount had increased markers of oxidative stress. It is concluded that n-3 fatty acids may be able to reverse the adverse metabolic effects induced by a high fructose diet
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marinedrugs-13-01864.pdf
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Accepted/In Press date: 23 March 2015
Published date: 1 April 2015
Keywords:
fish oil, fructose, metabolic syndrome, omega-3 fatty acids
Organisations:
Human Development & Health
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Local EPrints ID: 376552
URI: http://eprints.soton.ac.uk/id/eprint/376552
ISSN: 1660-3397
PURE UUID: 26e2df35-4170-49c1-9dee-23a2df526fb2
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Date deposited: 30 Apr 2015 08:29
Last modified: 15 Mar 2024 02:50
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Author:
G.S. de Castro
Author:
R. Deminice
Author:
L.M.C. Simoes-Ambrosio
Author:
A.A. Jordao
Author:
H. Vannucchi
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