Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide
Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide
In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the ?–? stacking interaction between the nucleobases of the DNA and the sp2 carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM.
12422-12429
Alonso-Cristobal, P.
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Vilela, P.
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El-Sagheer, A.
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Lopez-Cabarcos, E.
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Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Muskens, O.L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Rubio-Retama, J.
10c1f28b-f66b-4d66-aec8-0879414031bc
Kanaras, A.G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
17 June 2015
Alonso-Cristobal, P.
8badd4be-b605-40c9-8610-cb04cd326177
Vilela, P.
5948c49e-b217-49e6-b689-4e41264b4449
El-Sagheer, A.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Lopez-Cabarcos, E.
b717ce52-ba79-4727-82d7-e3c52d7a9cd0
Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Muskens, O.L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Rubio-Retama, J.
10c1f28b-f66b-4d66-aec8-0879414031bc
Kanaras, A.G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Alonso-Cristobal, P., Vilela, P., El-Sagheer, A., Lopez-Cabarcos, E., Brown, T., Muskens, O.L., Rubio-Retama, J. and Kanaras, A.G.
(2015)
Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide.
ACS Applied Materials and Interfaces, 7 (23), .
(doi:10.1021/am507591u).
Abstract
In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the ?–? stacking interaction between the nucleobases of the DNA and the sp2 carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM.
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Upconversion-DNA corrections R3N.docx
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Accepted/In Press date: 15 January 2015
e-pub ahead of print date: 27 January 2015
Published date: 17 June 2015
Organisations:
Quantum, Light & Matter Group
Identifiers
Local EPrints ID: 377404
URI: http://eprints.soton.ac.uk/id/eprint/377404
ISSN: 1944-8244
PURE UUID: c8b1a3cb-2764-4c0a-bdca-0521fcb687f1
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Date deposited: 11 Jun 2015 14:11
Last modified: 15 Mar 2024 03:34
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Contributors
Author:
P. Alonso-Cristobal
Author:
P. Vilela
Author:
A. El-Sagheer
Author:
E. Lopez-Cabarcos
Author:
T. Brown
Author:
J. Rubio-Retama
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