Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry


McCormack, Paul, Worsfold, Paul J. and Gledhill, Martha (2003) Separation and detection of siderophores produced by marine bacterioplankton using high-performance liquid chromatography with electrospray ionization mass spectrometry. Analytical Chemistry, 75, (11), 2647-2652. (doi:10.1021/ac0340105).

Download

Full text not available from this repository.

Original Publication URL: http://dx.doi.org/10.1021/ac0340105

Description/Abstract

High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.

Item Type: Article
ISSNs: 0003-2700 (print)
Related URLs:
Keywords: equatorial pacific-ocean, phytoplankton bloom, iron fertilization' desferrioxamine, speciation, sediments, seawater, bacteria
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
ePrint ID: 37741
Date Deposited: 25 May 2006
Last Modified: 27 Mar 2014 18:23
URI: http://eprints.soton.ac.uk/id/eprint/37741

Actions (login required)

View Item View Item