Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels
Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels
Microfluidic channels are investigated here with a view to unravel and deliver long genomic DNA strands (>20µm) into channels for subsequent optical interrogation. Previous studies have focused on devices where strands are stretched under sudden shear and elongational microflows, but unfortunately there is a potential for strand fragmentation. We investigate differently shaped microchannels to deliver intact strands for subsequent genome mapping.
microfluidics, DNA mapping, fluid dynamics, brownian motion
Horak, P.
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Noual, A.
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Humphreys, T.
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Melvin, T.
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January 2015
Horak, P.
520489b5-ccc7-4d29-bb30-c1e36436ea03
Noual, A.
5c5d5d71-3417-4e61-93fa-4b2c2bdc3513
Humphreys, T.
fc0792e4-e186-4fcd-a170-0ec11732d776
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Horak, P., Noual, A., Humphreys, T. and Melvin, T.
(2015)
Stretching of single DNA molecules under pressure-driven flow in straight and curved microfluidic channels.
Trends in Optical Micromanipulation III, , Obergurgl, Austria.
25 - 30 Jan 2015.
1 pp
.
Record type:
Conference or Workshop Item
(Paper)
Abstract
Microfluidic channels are investigated here with a view to unravel and deliver long genomic DNA strands (>20µm) into channels for subsequent optical interrogation. Previous studies have focused on devices where strands are stretched under sudden shear and elongational microflows, but unfortunately there is a potential for strand fragmentation. We investigate differently shaped microchannels to deliver intact strands for subsequent genome mapping.
More information
Published date: January 2015
Venue - Dates:
Trends in Optical Micromanipulation III, , Obergurgl, Austria, 2015-01-25 - 2015-01-30
Keywords:
microfluidics, DNA mapping, fluid dynamics, brownian motion
Organisations:
Optoelectronics Research Centre
Identifiers
Local EPrints ID: 379317
URI: http://eprints.soton.ac.uk/id/eprint/379317
PURE UUID: b5a9d4d9-a6f8-49fd-a1e4-a716be422f3f
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Date deposited: 21 Jul 2015 11:08
Last modified: 15 Mar 2024 03:13
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Contributors
Author:
A. Noual
Author:
T. Humphreys
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