Determination of phytochelatins and glutathione in phytoplankton from natural waters using HPLC with fluorescence detection


Kawakami, S.K., Gledhill, M. and Achterberg, E.P. (2006) Determination of phytochelatins and glutathione in phytoplankton from natural waters using HPLC with fluorescence detection. Trends in Analytical Chemistry, 25, (2), 133-142. (doi:10.1016/j.trac.2005.06.005).

Download

Full text not available from this repository.

Description/Abstract

Phytochelatins (PCs) and glutathione (GSH) are –SH-containing compounds produced by a range of organisms for intracellular functions, such as protection against oxidative stress, metal detoxification and regulation of intracellular metal concentrations. These compounds, particularly PCs, have a potential use as metal-stress indicators for phytoplankton in natural waters. Despite their important roles, there is a paucity of data on intracellular GSH and PCs produced by natural phytoplankton assemblages. Current analytical methods for the determination of these compounds in phytoplankton from natural waters are based on high-performance liquid chromatography (HPLC) with detection of fluorescent derivatives, and comprise multi-step protocols. In this article, we discuss the analytical methods for HPLC determination of PCs and GSH, as there are limitations and practical challenges when they are applied to environmental studies.

Item Type: Article
ISSNs: 0165-9936 (print)
Related URLs:
Keywords: glutathione; HPLC; metal detoxification; phytochelatin; thiol
Subjects: G Geography. Anthropology. Recreation > GC Oceanography
Divisions: University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
ePrint ID: 38077
Date Deposited: 01 Jun 2006
Last Modified: 27 Mar 2014 18:24
URI: http://eprints.soton.ac.uk/id/eprint/38077

Actions (login required)

View Item View Item