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The pattern of fatty acids displaced by EPA and DHA following 12 Months supplementation varies between blood cell and plasma fractions

The pattern of fatty acids displaced by EPA and DHA following 12 Months supplementation varies between blood cell and plasma fractions
The pattern of fatty acids displaced by EPA and DHA following 12 Months supplementation varies between blood cell and plasma fractions
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0-4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE) and triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and platelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases in several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA classes in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent manner in all pools after 12 months (37%-64% of placebo in the four portions group). We conclude that the profile of the FA decreased in exchange for the increase in EPA + DHA following supplementation differs by FA pool with implications for understanding the impact of n-3 PUFA on blood lipid and blood cell biology.
6281-6293
Walker, C.G.
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West, A.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Madden, J.
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Gambell, J.M.
a1518333-d6d2-4cce-85e6-6f1f438d0a70
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Walker, C.G.
612e7ef4-6bf9-42f6-9948-0ad00021fd49
West, A.
e8dacc1a-5fdc-4a4f-92d8-608f2ea2994c
Browning, L.M.
757097ae-415f-4f54-9e81-a129c5bcec83
Madden, J.
0771e352-d432-41ea-8a7e-4704c1efca46
Gambell, J.M.
a1518333-d6d2-4cce-85e6-6f1f438d0a70
Jebb, S.A.
c734720d-52eb-4656-a712-24398ef662f2
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Walker, C.G., West, A., Browning, L.M., Madden, J., Gambell, J.M., Jebb, S.A. and Calder, P.C. (2015) The pattern of fatty acids displaced by EPA and DHA following 12 Months supplementation varies between blood cell and plasma fractions. Nutrients, 7 (8), 6281-6293. (doi:10.3390/nu7085285). (PMID:26247960)

Record type: Article

Abstract

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0-4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE) and triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and platelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases in several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA classes in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent manner in all pools after 12 months (37%-64% of placebo in the four portions group). We conclude that the profile of the FA decreased in exchange for the increase in EPA + DHA following supplementation differs by FA pool with implications for understanding the impact of n-3 PUFA on blood lipid and blood cell biology.

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Published date: 3 August 2015
Organisations: Human Development & Health

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Local EPrints ID: 381152
URI: http://eprints.soton.ac.uk/id/eprint/381152
PURE UUID: 841a3f96-6022-4696-a80a-af034e00ad84
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 23 Sep 2015 15:13
Last modified: 15 Mar 2024 02:50

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Contributors

Author: C.G. Walker
Author: A. West
Author: L.M. Browning
Author: J. Madden
Author: J.M. Gambell
Author: S.A. Jebb
Author: P.C. Calder ORCID iD

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