Improved recovery of Listeria monocytogenes from stainless steel and polytetrafluoroethylene surfaces using air/water ablation
Improved recovery of Listeria monocytogenes from stainless steel and polytetrafluoroethylene surfaces using air/water ablation
Aims: to develop a gentle ablation technique to recover Listeria monocytogenes biofilms from stainless steel (SS) and polytetrafluoroethylene (PTFE) surfaces by using compressed air and water injection.
Methods and results: biofilms were grown for 4, 24 and 48 h or 7 days and a compressed air and water flow at 2, 3 and 4 bars was applied for cell removal. Collected cells were quantified for total/dead by staining with SYTO 9/PI double staining and cultivable populations were determined by plating onto brain heart infusion (BHI) agar, while coupon surfaces also were stained with DAPI to quantify in situ the remaining cells. The recovery efficiency was compared to that of conventional swabbing. Results showed that the air/water ablation is able to collect up to 98·6% of cells from SS surfaces while swabbing only recovered 11·2% of biofilm. Moreover, air/water ablation recovered 99·9% of cells from PTFE surfaces.
Conclusions: the high recovery rate achieved by this technique, along with the fact that cells were able to retain membrane integrity and cultivability, indicate that this device is suitable for the gentle recovery of viable L. monocytogenes biofilm cells.
Significance and impact of the study: this work presents a highly efficient technique to remove, collect and quantify L. monocytogenes from surfaces commonly used in the food industry, which can thus serve as an important aid in verifying cleaning and sanitation as well as in reducing the likelihood of cross-contamination events
air/water ablation, biofilms recovery, listeria monocytogenes, polytetrafluoroethylene surfaces, stainless steel surfaces
253-262
Giao, M.S.
5638b770-3681-48b2-a9ae-9152b36ac504
Blanc, Severine
46d6d12b-6fb5-49a7-92df-f0654c2f5260
Porta, Sonia
301d5421-99f0-4655-92e7-a9484244c445
Belenguer, Jose
a8ca963b-3979-4c2d-a2a1-27c73cea5eb5
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
July 2015
Giao, M.S.
5638b770-3681-48b2-a9ae-9152b36ac504
Blanc, Severine
46d6d12b-6fb5-49a7-92df-f0654c2f5260
Porta, Sonia
301d5421-99f0-4655-92e7-a9484244c445
Belenguer, Jose
a8ca963b-3979-4c2d-a2a1-27c73cea5eb5
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Giao, M.S., Blanc, Severine, Porta, Sonia, Belenguer, Jose and Keevil, C.W.
(2015)
Improved recovery of Listeria monocytogenes from stainless steel and polytetrafluoroethylene surfaces using air/water ablation.
Journal of Applied Microbiology, 119, .
(doi:10.1111/jam.12837).
Abstract
Aims: to develop a gentle ablation technique to recover Listeria monocytogenes biofilms from stainless steel (SS) and polytetrafluoroethylene (PTFE) surfaces by using compressed air and water injection.
Methods and results: biofilms were grown for 4, 24 and 48 h or 7 days and a compressed air and water flow at 2, 3 and 4 bars was applied for cell removal. Collected cells were quantified for total/dead by staining with SYTO 9/PI double staining and cultivable populations were determined by plating onto brain heart infusion (BHI) agar, while coupon surfaces also were stained with DAPI to quantify in situ the remaining cells. The recovery efficiency was compared to that of conventional swabbing. Results showed that the air/water ablation is able to collect up to 98·6% of cells from SS surfaces while swabbing only recovered 11·2% of biofilm. Moreover, air/water ablation recovered 99·9% of cells from PTFE surfaces.
Conclusions: the high recovery rate achieved by this technique, along with the fact that cells were able to retain membrane integrity and cultivability, indicate that this device is suitable for the gentle recovery of viable L. monocytogenes biofilm cells.
Significance and impact of the study: this work presents a highly efficient technique to remove, collect and quantify L. monocytogenes from surfaces commonly used in the food industry, which can thus serve as an important aid in verifying cleaning and sanitation as well as in reducing the likelihood of cross-contamination events
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More information
Accepted/In Press date: 24 April 2015
Published date: July 2015
Keywords:
air/water ablation, biofilms recovery, listeria monocytogenes, polytetrafluoroethylene surfaces, stainless steel surfaces
Organisations:
Centre for Biological Sciences
Identifiers
Local EPrints ID: 383142
URI: http://eprints.soton.ac.uk/id/eprint/383142
ISSN: 1364-5072
PURE UUID: 79487eaf-68f8-4756-8537-a40e8eed3235
Catalogue record
Date deposited: 06 Nov 2015 16:22
Last modified: 15 Mar 2024 03:12
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Contributors
Author:
Severine Blanc
Author:
Sonia Porta
Author:
Jose Belenguer
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