Using electrochemical SERS to measure the redox potential of drug molecules bound to dsDNA - a study of mitoxantrone
Using electrochemical SERS to measure the redox potential of drug molecules bound to dsDNA - a study of mitoxantrone
Interaction with DNA plays an important role in the biological activity of some anticancer drug molecules. In this paper we show that electrochemical surface enhanced Raman spectroscopy at sphere segment void gold electrodes can be used as a highly sensitive technique to measure the redox potential of the anticancer drug mitoxantrone bound to dsDNA. For this system we show that we can follow the redox reaction of the bound molecule and can extract the redox potential for the molecule bound to dsDNA by deconvolution of the SER spectra recorded as a function of electrode potential. We find that mitoxantrone bound to dsDNA undergoes a 2 electron, 1 proton reduction and that the redox potential (-0.87 V vs. Ag/AgCl at pH 7.2) is shifted approximately 0.12 V cathodic of the corresponding value at a glassy carbon electrode. Our results also show that the reduced form of mitoxantrone remains bound to dsDNA and we are able to use the deconvoluted SER spectra of the reduced mitoxantrone as a function of electrode potential to follow the electrochemically driven melting of the dsDNA at more negative potentials.
electrochemical SERS, mitoxantrone, dsDNA, redox reaction
684-692
Meneghello, Marta
0978ae7b-821d-49d9-9dac-a2a8e2306d5b
Papadopoulou, Evanthia
235d2d8f-e2af-4fc0-a402-5fc36184f621
Ugo, Paolo
6cb120fd-9f8f-4104-bc5e-7345bc3cd88d
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
1 January 2016
Meneghello, Marta
0978ae7b-821d-49d9-9dac-a2a8e2306d5b
Papadopoulou, Evanthia
235d2d8f-e2af-4fc0-a402-5fc36184f621
Ugo, Paolo
6cb120fd-9f8f-4104-bc5e-7345bc3cd88d
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Meneghello, Marta, Papadopoulou, Evanthia, Ugo, Paolo and Bartlett, Philip N.
(2016)
Using electrochemical SERS to measure the redox potential of drug molecules bound to dsDNA - a study of mitoxantrone.
Electrochimica Acta, 187, .
(doi:10.1016/j.electacta.2015.11.121).
Abstract
Interaction with DNA plays an important role in the biological activity of some anticancer drug molecules. In this paper we show that electrochemical surface enhanced Raman spectroscopy at sphere segment void gold electrodes can be used as a highly sensitive technique to measure the redox potential of the anticancer drug mitoxantrone bound to dsDNA. For this system we show that we can follow the redox reaction of the bound molecule and can extract the redox potential for the molecule bound to dsDNA by deconvolution of the SER spectra recorded as a function of electrode potential. We find that mitoxantrone bound to dsDNA undergoes a 2 electron, 1 proton reduction and that the redox potential (-0.87 V vs. Ag/AgCl at pH 7.2) is shifted approximately 0.12 V cathodic of the corresponding value at a glassy carbon electrode. Our results also show that the reduced form of mitoxantrone remains bound to dsDNA and we are able to use the deconvoluted SER spectra of the reduced mitoxantrone as a function of electrode potential to follow the electrochemically driven melting of the dsDNA at more negative potentials.
Text
ECA MTX_v6 revised.pdf
- Accepted Manuscript
More information
Accepted/In Press date: 24 November 2015
e-pub ahead of print date: 2 December 2015
Published date: 1 January 2016
Keywords:
electrochemical SERS, mitoxantrone, dsDNA, redox reaction
Organisations:
Electrochemistry
Identifiers
Local EPrints ID: 385453
URI: http://eprints.soton.ac.uk/id/eprint/385453
ISSN: 0013-4686
PURE UUID: 1f64cee6-bc07-4ef8-a4de-0c3fb60cc1c5
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Date deposited: 20 Jan 2016 09:25
Last modified: 15 Mar 2024 02:44
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Contributors
Author:
Marta Meneghello
Author:
Evanthia Papadopoulou
Author:
Paolo Ugo
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