Low cost thin-film transistor nanoribbon sensors for
detection of proteins using a miniature bead-based
enzyme-linked immunosorbent assay (elisa)
Low cost thin-film transistor nanoribbon sensors for
detection of proteins using a miniature bead-based
enzyme-linked immunosorbent assay (elisa)
We demonstrate a low cost thin-film transistor (TFT) nanoribbon sensor for measuring enzyme-substrate reactions. The sensor is used to measure the inflammatory biomarker C-reactive protein (CRP) in human serum using a miniature bead-based Enzyme-Linked Immunosorbent Assay (ELISA) with the sensor measuring pH. Rather than binding antibodies directly to the sensor surface, a magnetic bead-based ELISA was used. This keeps the functionalization steps and capture moieties away from the sensor surface, increasing the binding sites and improving speed and sensitivity. The ability to sense proteins in physiological buffer via enzyme activity overcomes the Debye length limitation associated with nano-biosensors.
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis, I.
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
2ace53c1-82cc-47e5-911e-898b143163d3
Ashburn, Peter
391bdd4d-acd3-4a4f-ab1b-874492c71380
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
October 2015
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis, I.
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
2ace53c1-82cc-47e5-911e-898b143163d3
Ashburn, Peter
391bdd4d-acd3-4a4f-ab1b-874492c71380
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hu, Chunxiao, Zeimpekis, I., Sun, Kai, Ashburn, Peter and Morgan, Hywel
(2015)
Low cost thin-film transistor nanoribbon sensors for
detection of proteins using a miniature bead-based
enzyme-linked immunosorbent assay (elisa).
MicroTAS 2015, Gyeongju, Korea, Republic of.
25 - 29 Oct 2015.
3 pp
.
Record type:
Conference or Workshop Item
(Paper)
Abstract
We demonstrate a low cost thin-film transistor (TFT) nanoribbon sensor for measuring enzyme-substrate reactions. The sensor is used to measure the inflammatory biomarker C-reactive protein (CRP) in human serum using a miniature bead-based Enzyme-Linked Immunosorbent Assay (ELISA) with the sensor measuring pH. Rather than binding antibodies directly to the sensor surface, a magnetic bead-based ELISA was used. This keeps the functionalization steps and capture moieties away from the sensor surface, increasing the binding sites and improving speed and sensitivity. The ability to sense proteins in physiological buffer via enzyme activity overcomes the Debye length limitation associated with nano-biosensors.
Text
MicroTAS2015_Manuscript_Chunxiao Hu.pdf
- Accepted Manuscript
More information
Accepted/In Press date: 30 July 2015
Published date: October 2015
Venue - Dates:
MicroTAS 2015, Gyeongju, Korea, Republic of, 2015-10-25 - 2015-10-29
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 386171
URI: http://eprints.soton.ac.uk/id/eprint/386171
PURE UUID: bf6470e0-b2b1-49fa-a6bd-ea083fda3b9f
Catalogue record
Date deposited: 01 Feb 2016 11:46
Last modified: 15 Mar 2024 03:38
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Contributors
Author:
Chunxiao Hu
Author:
Kai Sun
Author:
Peter Ashburn
Author:
Hywel Morgan
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