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Development and characterization of monoclonal antibodies specific for mouse and human Fc? receptors

Development and characterization of monoclonal antibodies specific for mouse and human Fc? receptors
Development and characterization of monoclonal antibodies specific for mouse and human Fc? receptors
Fc?Rs are key regulators of the immune response, capable of binding to the Fc portion of IgG Abs and manipulating the behavior of numerous cell types. Through a variety of receptors, isoforms, and cellular expression patterns, they are able to fine-tune and direct appropriate responses. Furthermore, they are key determinants of mAb immunotherapy, with mAb isotype and Fc?R interaction governing therapeutic efficacy. Critical to understanding the biology of this complex family of receptors are reagents that are robust and highly specific for each receptor. In this study, we describe the development and characterization of mAb panels specific for both mouse and human Fc?R for use in flow cytometry, immunofluorescence, and immunocytochemistry. We highlight key differences in expression between the two species and also patterns of expression that will likely impact on immunotherapeutic efficacy and translation of therapeutic agents from mouse to clinic.
0022-1767
5503-5516
Tutt, A.
46ce577b-aea1-412d-84ea-fc4dab794469
James, S.
764c80e3-5bea-4b34-a871-b43f87ef97b0
Laversin, S.A.
e9122da5-bfb0-4260-b7db-659fb855e898
Tipton, T.R.W.
a0a94b14-7d09-4411-b988-84c9d39eb0d6
Ashton-Key, M.
5111ac18-7d4f-4ef0-9c71-0a44c37aaed4
French, R.R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Hussain, K.
9468f252-81d0-4251-b800-702433b610f8
Vaughan, A. T.
bfb2ceab-a592-457e-89f9-00fcd1dddbdb
Dou, L.
78292b26-0ece-40d8-85fd-d24661c3ca15
Earley, A.
aa795fdf-fd74-474c-91ad-5c2bd597213a
Dahal, L.N.
1e993a7a-b007-4187-82ea-e28dd3920b66
Lu, C.
456c6d86-4b34-4ba7-917c-83ebf1f4c08b
Dunscombe, M.
4b6f0274-89a2-423b-8094-0cc64ffe3722
Chan, H.T.C.
b109c93f-7e9a-44ee-ad12-da757b1b11fc
Penfold, C.A.
400d743e-a639-45ea-a027-5b778800f6d3
Kim, J.H.
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Potter, E.A.
7828a574-b547-4955-961f-a4a17ed9d491
Mockridge, C.I.
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Roghanian, A.
e2b032c2-60a0-4522-a3d8-56a768792f36
Oldham, R.J.
f43d1416-0b93-4dfd-a504-2850cb43e87e
Cox, K.L.
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Lim, S.H.
1afe5aa1-61a4-4a7b-927f-5e671f885196
Teige, I.
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Frendeus, B.
ea6161a4-2041-4317-9802-95f623219fa6
Glennie, M.J.
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Beers, S.A.
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Cragg, M.S.
ec97f80e-f3c8-49b7-a960-20dff648b78c
Tutt, A.
46ce577b-aea1-412d-84ea-fc4dab794469
James, S.
764c80e3-5bea-4b34-a871-b43f87ef97b0
Laversin, S.A.
e9122da5-bfb0-4260-b7db-659fb855e898
Tipton, T.R.W.
a0a94b14-7d09-4411-b988-84c9d39eb0d6
Ashton-Key, M.
5111ac18-7d4f-4ef0-9c71-0a44c37aaed4
French, R.R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Hussain, K.
9468f252-81d0-4251-b800-702433b610f8
Vaughan, A. T.
bfb2ceab-a592-457e-89f9-00fcd1dddbdb
Dou, L.
78292b26-0ece-40d8-85fd-d24661c3ca15
Earley, A.
aa795fdf-fd74-474c-91ad-5c2bd597213a
Dahal, L.N.
1e993a7a-b007-4187-82ea-e28dd3920b66
Lu, C.
456c6d86-4b34-4ba7-917c-83ebf1f4c08b
Dunscombe, M.
4b6f0274-89a2-423b-8094-0cc64ffe3722
Chan, H.T.C.
b109c93f-7e9a-44ee-ad12-da757b1b11fc
Penfold, C.A.
400d743e-a639-45ea-a027-5b778800f6d3
Kim, J.H.
449c77b0-dc96-425b-8fad-4b5e214fa93f
Potter, E.A.
7828a574-b547-4955-961f-a4a17ed9d491
Mockridge, C.I.
327aef17-4837-4f2a-a93b-3d17cd1a7f9f
Roghanian, A.
e2b032c2-60a0-4522-a3d8-56a768792f36
Oldham, R.J.
f43d1416-0b93-4dfd-a504-2850cb43e87e
Cox, K.L.
7305c27e-9cdc-4e37-b994-ac55d7d1dfd2
Lim, S.H.
1afe5aa1-61a4-4a7b-927f-5e671f885196
Teige, I.
2f8d7623-431d-4c93-85a4-b36e367a518f
Frendeus, B.
ea6161a4-2041-4317-9802-95f623219fa6
Glennie, M.J.
9f6f0eff-4560-48c2-80cd-0ec116110ded
Beers, S.A.
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Cragg, M.S.
ec97f80e-f3c8-49b7-a960-20dff648b78c

Tutt, A., James, S., Laversin, S.A., Tipton, T.R.W., Ashton-Key, M., French, R.R., Hussain, K., Vaughan, A. T., Dou, L., Earley, A., Dahal, L.N., Lu, C., Dunscombe, M., Chan, H.T.C., Penfold, C.A., Kim, J.H., Potter, E.A., Mockridge, C.I., Roghanian, A., Oldham, R.J., Cox, K.L., Lim, S.H., Teige, I., Frendeus, B., Glennie, M.J., Beers, S.A. and Cragg, M.S. (2015) Development and characterization of monoclonal antibodies specific for mouse and human Fc? receptors. Journal of Immunology, 195 (11), 5503-5516. (doi:10.4049/jimmunol.1402988). (PMID:26512139)

Record type: Article

Abstract

Fc?Rs are key regulators of the immune response, capable of binding to the Fc portion of IgG Abs and manipulating the behavior of numerous cell types. Through a variety of receptors, isoforms, and cellular expression patterns, they are able to fine-tune and direct appropriate responses. Furthermore, they are key determinants of mAb immunotherapy, with mAb isotype and Fc?R interaction governing therapeutic efficacy. Critical to understanding the biology of this complex family of receptors are reagents that are robust and highly specific for each receptor. In this study, we describe the development and characterization of mAb panels specific for both mouse and human Fc?R for use in flow cytometry, immunofluorescence, and immunocytochemistry. We highlight key differences in expression between the two species and also patterns of expression that will likely impact on immunotherapeutic efficacy and translation of therapeutic agents from mouse to clinic.

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More information

Accepted/In Press date: 30 September 2015
e-pub ahead of print date: 28 October 2015
Published date: 1 December 2015
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 390176
URI: http://eprints.soton.ac.uk/id/eprint/390176
ISSN: 0022-1767
PURE UUID: 6149265e-a169-4265-853a-c975f851cb7e
ORCID for A. T. Vaughan: ORCID iD orcid.org/0000-0001-6076-3649
ORCID for H.T.C. Chan: ORCID iD orcid.org/0000-0003-0530-9480
ORCID for A. Roghanian: ORCID iD orcid.org/0000-0003-1316-4218
ORCID for S.A. Beers: ORCID iD orcid.org/0000-0002-3765-3342
ORCID for M.S. Cragg: ORCID iD orcid.org/0000-0003-2077-089X

Catalogue record

Date deposited: 21 Mar 2016 16:35
Last modified: 15 Mar 2024 03:34

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Contributors

Author: A. Tutt
Author: S. James
Author: S.A. Laversin
Author: T.R.W. Tipton
Author: M. Ashton-Key
Author: R.R. French
Author: K. Hussain
Author: A. T. Vaughan ORCID iD
Author: L. Dou
Author: A. Earley
Author: L.N. Dahal
Author: C. Lu
Author: M. Dunscombe
Author: H.T.C. Chan ORCID iD
Author: C.A. Penfold
Author: J.H. Kim
Author: E.A. Potter
Author: C.I. Mockridge
Author: A. Roghanian ORCID iD
Author: R.J. Oldham
Author: K.L. Cox
Author: S.H. Lim
Author: I. Teige
Author: B. Frendeus
Author: M.J. Glennie
Author: S.A. Beers ORCID iD
Author: M.S. Cragg ORCID iD

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