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Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function

Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function
Background: endoglin/CD105 is an auxiliary receptor for transforming growth factor-? with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study.

Results: in vitro culture of mouse islets with VEGF164 for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture.

Conclusion: EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size
1756-0500
Clarkin, Claire
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Mahmoud, Marwa
c9aac4f7-b421-433a-baf4-e173ae5a0fc0
Liu, Bo
b4376e27-629b-4700-b104-3548c71f35f8
Sobamowo, Emmanuel O.
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King, Aileen
b4ecd67e-6d3b-4908-8eb7-f98fc7121d1b
Arthur, Helen
89c8e03f-2ad4-41be-a1ff-1aa68618c531
Jones, Peter M.
38a5ea85-3372-4389-bbf0-f4330dd86f6d
Wheeler-Jones, Caroline P.
c5bcaf30-9554-4c43-82d9-dbcdc1cac981
Clarkin, Claire
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Mahmoud, Marwa
c9aac4f7-b421-433a-baf4-e173ae5a0fc0
Liu, Bo
b4376e27-629b-4700-b104-3548c71f35f8
Sobamowo, Emmanuel O.
8c3b1d80-1c27-4138-a5f2-e5384a65c27d
King, Aileen
b4ecd67e-6d3b-4908-8eb7-f98fc7121d1b
Arthur, Helen
89c8e03f-2ad4-41be-a1ff-1aa68618c531
Jones, Peter M.
38a5ea85-3372-4389-bbf0-f4330dd86f6d
Wheeler-Jones, Caroline P.
c5bcaf30-9554-4c43-82d9-dbcdc1cac981

Clarkin, Claire, Mahmoud, Marwa, Liu, Bo, Sobamowo, Emmanuel O., King, Aileen, Arthur, Helen, Jones, Peter M. and Wheeler-Jones, Caroline P. (2016) Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function. BMC Research Notes, 9 (1). (doi:10.1186/s13104-016-2142-z).

Record type: Article

Abstract

Background: endoglin/CD105 is an auxiliary receptor for transforming growth factor-? with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study.

Results: in vitro culture of mouse islets with VEGF164 for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture.

Conclusion: EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size

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Accepted/In Press date: 30 June 2016
e-pub ahead of print date: 25 July 2016
Organisations: Biomedicine

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Local EPrints ID: 398576
URI: http://eprints.soton.ac.uk/id/eprint/398576
ISSN: 1756-0500
PURE UUID: d75f8979-3848-4f40-b96e-6750bde5fc02

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Date deposited: 28 Jul 2016 10:49
Last modified: 15 Mar 2024 01:36

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Contributors

Author: Claire Clarkin
Author: Marwa Mahmoud
Author: Bo Liu
Author: Emmanuel O. Sobamowo
Author: Aileen King
Author: Helen Arthur
Author: Peter M. Jones
Author: Caroline P. Wheeler-Jones

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