The University of Southampton
University of Southampton Institutional Repository

Primary air–liquid interface culture of nasal epithelium for nasal drug delivery

Primary air–liquid interface culture of nasal epithelium for nasal drug delivery
Primary air–liquid interface culture of nasal epithelium for nasal drug delivery
Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air–liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.
1543-8384
2242-2252
Ong, Hui Xin
f2241b82-9866-4737-afdf-3e99faa43c60
Jackson, Claire L.
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Cole, Janice L.
fb9d20aa-93b9-42b3-9b9e-bab2f565ea60
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Traini, Daniela
ed203afd-8e53-427c-a473-3c1cd26c23ea
Young, Paul M.
17827d88-bd0f-470b-af66-88f73b378917
Lucas, Jane
5cb3546c-87b2-4e59-af48-402076e25313
Conway, Joy
bbe9a2e4-fb85-4d4a-a38c-0c1832c32d06
Ong, Hui Xin
f2241b82-9866-4737-afdf-3e99faa43c60
Jackson, Claire L.
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Cole, Janice L.
fb9d20aa-93b9-42b3-9b9e-bab2f565ea60
Lackie, Peter M.
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Traini, Daniela
ed203afd-8e53-427c-a473-3c1cd26c23ea
Young, Paul M.
17827d88-bd0f-470b-af66-88f73b378917
Lucas, Jane
5cb3546c-87b2-4e59-af48-402076e25313
Conway, Joy
bbe9a2e4-fb85-4d4a-a38c-0c1832c32d06

Ong, Hui Xin, Jackson, Claire L., Cole, Janice L., Lackie, Peter M., Traini, Daniela, Young, Paul M., Lucas, Jane and Conway, Joy (2016) Primary air–liquid interface culture of nasal epithelium for nasal drug delivery. Molecular Pharmaceutics, 13 (7), 2242-2252. (doi:10.1021/acs.molpharmaceut.5b00852).

Record type: Article

Abstract

Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air–liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.

Text
Primary air liquid interface.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

Accepted/In Press date: 25 May 2016
e-pub ahead of print date: 25 May 2016
Published date: 5 July 2016
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 399414
URI: http://eprints.soton.ac.uk/id/eprint/399414
ISSN: 1543-8384
PURE UUID: 11e49de9-c221-4bb1-b945-fd1458839359
ORCID for Claire L. Jackson: ORCID iD orcid.org/0000-0002-1200-0935
ORCID for Peter M. Lackie: ORCID iD orcid.org/0000-0001-7138-3764
ORCID for Jane Lucas: ORCID iD orcid.org/0000-0001-8701-9975
ORCID for Joy Conway: ORCID iD orcid.org/0000-0001-6464-1526

Catalogue record

Date deposited: 16 Aug 2016 08:58
Last modified: 15 Mar 2024 03:22

Export record

Altmetrics

Contributors

Author: Hui Xin Ong
Author: Claire L. Jackson ORCID iD
Author: Janice L. Cole
Author: Peter M. Lackie ORCID iD
Author: Daniela Traini
Author: Paul M. Young
Author: Jane Lucas ORCID iD
Author: Joy Conway ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×