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Ultrasound-enhanced siRNA delivery using magnetic nanoparticle-loaded chitosan-deoxycholic acid nanodroplets

Ultrasound-enhanced siRNA delivery using magnetic nanoparticle-loaded chitosan-deoxycholic acid nanodroplets
Ultrasound-enhanced siRNA delivery using magnetic nanoparticle-loaded chitosan-deoxycholic acid nanodroplets
Small interfering RNA (siRNA) has significant therapeutic potential but its clinical translation has been severely inhibited by a lack of effective delivery strategies. Previous work has demonstrated that perfluorocarbon nanodroplets loaded with magnetic nanoparticles can facilitate the intracellular delivery of a conventional chemotherapeutic drug. The aim of this study was to determine whether a similar agent could provide a means of delivering siRNA, enabling efficient transfection without degradation of the molecule. Chitosan-deoxycholic acid nanoparticles containing perfluoropentane and iron oxide (d0 = 7.5 ± 0.35 nm) with a mean hydrodynamic diameter of 257.6 ± 10.9 nm were produced. siRNA (AllStars Hs cell death siRNA) was electrostatically bound to the particle surface and delivery to lung cancer cells and breast cancer cells was investigated with and without ultrasound exposure (500 kHz, 1 MPa peak-to-peak focal pressure, 40 cycles per burst, 1 kHz PRF, 10 seconds duration). The results showed that siRNA functionality was not impaired by the treatment protocol and that the nanodroplets were able to successfully promote siRNA uptake, leading to significant apoptosis (52.4%) 72 hours after ultrasound treatment.
2192-2659
1-9
Yu Lee, Jeong
888f4a41-2dc6-4ae9-bf86-da51e728b94e
Crake, Calum
ab3eeead-4851-4c54-bea9-383cf136c4bb
Teo, Boon
6b8c4e26-0544-4055-ac7d-85b155858047
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Seth, Anjali
92f275f5-ebf6-4e76-9c16-d8438f765ca2
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Yu Lee, Jeong
888f4a41-2dc6-4ae9-bf86-da51e728b94e
Crake, Calum
ab3eeead-4851-4c54-bea9-383cf136c4bb
Teo, Boon
6b8c4e26-0544-4055-ac7d-85b155858047
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Seth, Anjali
92f275f5-ebf6-4e76-9c16-d8438f765ca2
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Yu Lee, Jeong, Crake, Calum, Teo, Boon, Carugo, Dario, de Saint Victor, Marie, Seth, Anjali and Stride, Eleanor (2017) Ultrasound-enhanced siRNA delivery using magnetic nanoparticle-loaded chitosan-deoxycholic acid nanodroplets. Advanced Healthcare Materials, 6 (8), 1-9, [1601246]. (doi:10.1002/adhm.201601246).

Record type: Article

Abstract

Small interfering RNA (siRNA) has significant therapeutic potential but its clinical translation has been severely inhibited by a lack of effective delivery strategies. Previous work has demonstrated that perfluorocarbon nanodroplets loaded with magnetic nanoparticles can facilitate the intracellular delivery of a conventional chemotherapeutic drug. The aim of this study was to determine whether a similar agent could provide a means of delivering siRNA, enabling efficient transfection without degradation of the molecule. Chitosan-deoxycholic acid nanoparticles containing perfluoropentane and iron oxide (d0 = 7.5 ± 0.35 nm) with a mean hydrodynamic diameter of 257.6 ± 10.9 nm were produced. siRNA (AllStars Hs cell death siRNA) was electrostatically bound to the particle surface and delivery to lung cancer cells and breast cancer cells was investigated with and without ultrasound exposure (500 kHz, 1 MPa peak-to-peak focal pressure, 40 cycles per burst, 1 kHz PRF, 10 seconds duration). The results showed that siRNA functionality was not impaired by the treatment protocol and that the nanodroplets were able to successfully promote siRNA uptake, leading to significant apoptosis (52.4%) 72 hours after ultrasound treatment.

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More information

Accepted/In Press date: 10 January 2017
e-pub ahead of print date: 13 February 2017
Published date: 19 April 2017
Organisations: Bioengineering Group, Mechatronics

Identifiers

Local EPrints ID: 404601
URI: http://eprints.soton.ac.uk/id/eprint/404601
ISSN: 2192-2659
PURE UUID: 76ac53f8-ee42-4cef-a8ae-2024df589076

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Date deposited: 12 Jan 2017 11:49
Last modified: 15 Mar 2024 06:12

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Contributors

Author: Jeong Yu Lee
Author: Calum Crake
Author: Boon Teo
Author: Dario Carugo
Author: Marie de Saint Victor
Author: Anjali Seth
Author: Eleanor Stride

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