The University of Southampton
University of Southampton Institutional Repository

Effect of parenteral infusion of fish oil-based lipid emulsion on systemic inflammatory cytokines and lung eicosanoid levels in experimental acute pancreatitis

Effect of parenteral infusion of fish oil-based lipid emulsion on systemic inflammatory cytokines and lung eicosanoid levels in experimental acute pancreatitis
Effect of parenteral infusion of fish oil-based lipid emulsion on systemic inflammatory cytokines and lung eicosanoid levels in experimental acute pancreatitis
Aim: we evaluated the effect of short-term FOLE infusion before experimental induction of AP on systemic cytokine and lung eicosanoid profiles.Methods: Lewis rats (n = 72) received parenteral infusion of FOLE (FO group) or saline (SS group), or remained without parenteral infusion (CG group) for 48 h. Thereafter, AP was induced by retrograde injection of sodium taurocholate into the pancreatic duct. Animals were sacrificed after 2, 12 and 24 h. Blood and lung samples were collected to assess serum inflammatory cytokines (Luminex) and tissue eicosanoids (ELISA), respectively.Results: serum TNF-α increased over time and serum IL-10 decreased from 12 to 24 h in CG group. In SS group serum TNF-α increased from 12 to 24 h (p = 0.039) and serum IL-10 decreased over time. Both CG and SS groups exhibited increased IL-6/IL-10 ratio (p = 0.040). From 12 to 24 h animals from FO group showed decreased serum IL-1 (p < 0.001), IL-4 (p < 0.002) and IL-6 (p = 0.050), and a trend towards increased IL-10 (p = 0.060). All experimental groups showed a trend towards increased PGE2 and decreased LTB4 in the lung at 24 compared with 12 hConclusion: parenteral infusion of FOLE for 48 h before the induction of experimental AP appears to favorably influence the cytokine response without affecting lung eicosanoids at the time points measured. The use of FOLE to prevent and treat AP following major surgery needs to be further explored
0261-5614
302
Garla, Priscila
0a5e65ca-8d4e-4344-947d-5c1f2a6970c4
Garib, R
2d5ed58c-10e2-4651-ac66-22e7cd054905
Torrinhas, R.S.
bc3c42e4-1856-47a8-a17f-aac863645ad4
Machado, M.C.
7ed10dbb-180d-4cdd-ae8f-0b05b4eb518d
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Waitzberg, D.L.
58babb59-3daa-4346-b2c8-6eecf7dd81a7
Garla, Priscila
0a5e65ca-8d4e-4344-947d-5c1f2a6970c4
Garib, R
2d5ed58c-10e2-4651-ac66-22e7cd054905
Torrinhas, R.S.
bc3c42e4-1856-47a8-a17f-aac863645ad4
Machado, M.C.
7ed10dbb-180d-4cdd-ae8f-0b05b4eb518d
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Waitzberg, D.L.
58babb59-3daa-4346-b2c8-6eecf7dd81a7

Garla, Priscila, Garib, R, Torrinhas, R.S., Machado, M.C., Calder, Philip and Waitzberg, D.L. (2017) Effect of parenteral infusion of fish oil-based lipid emulsion on systemic inflammatory cytokines and lung eicosanoid levels in experimental acute pancreatitis. Clinical Nutrition, 36 (1), 302. (doi:10.1016/j.clnu.2015.12.006).

Record type: Article

Abstract

Aim: we evaluated the effect of short-term FOLE infusion before experimental induction of AP on systemic cytokine and lung eicosanoid profiles.Methods: Lewis rats (n = 72) received parenteral infusion of FOLE (FO group) or saline (SS group), or remained without parenteral infusion (CG group) for 48 h. Thereafter, AP was induced by retrograde injection of sodium taurocholate into the pancreatic duct. Animals were sacrificed after 2, 12 and 24 h. Blood and lung samples were collected to assess serum inflammatory cytokines (Luminex) and tissue eicosanoids (ELISA), respectively.Results: serum TNF-α increased over time and serum IL-10 decreased from 12 to 24 h in CG group. In SS group serum TNF-α increased from 12 to 24 h (p = 0.039) and serum IL-10 decreased over time. Both CG and SS groups exhibited increased IL-6/IL-10 ratio (p = 0.040). From 12 to 24 h animals from FO group showed decreased serum IL-1 (p < 0.001), IL-4 (p < 0.002) and IL-6 (p = 0.050), and a trend towards increased IL-10 (p = 0.060). All experimental groups showed a trend towards increased PGE2 and decreased LTB4 in the lung at 24 compared with 12 hConclusion: parenteral infusion of FOLE for 48 h before the induction of experimental AP appears to favorably influence the cytokine response without affecting lung eicosanoids at the time points measured. The use of FOLE to prevent and treat AP following major surgery needs to be further explored

Text
Final FOLE clinical nutrition 21122015 - Accepted Manuscript
Download (556kB)

More information

Accepted/In Press date: 10 December 2015
Published date: 1 February 2017
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 406324
URI: http://eprints.soton.ac.uk/id/eprint/406324
ISSN: 0261-5614
PURE UUID: 4c27c453-93f3-4bad-8c6f-9d0de24a3765
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 10 Mar 2017 10:44
Last modified: 16 Mar 2024 02:51

Export record

Altmetrics

Contributors

Author: Priscila Garla
Author: R Garib
Author: R.S. Torrinhas
Author: M.C. Machado
Author: Philip Calder ORCID iD
Author: D.L. Waitzberg

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×