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In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles

In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles
In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles
Activating the immune system to trigger a specific response is a major challenge in vaccine development. In particular, activating sufficient cytotoxic T lymphocyte-mediated cellular immunity, which is crucial for the treatment of many diseases including cancer and AIDS, has proven to be especially challenging. In this study, antigens were encapsulated in acid-degradable polymeric particle carriers to cascade cytotoxic T lymphocyte activation. To target dendritic cells, the most potent antigen-presenting cells, the particle carriers, were further conjugated with monoclonal antibodies. A series of ex vivo and in vivo studies have shown increased receptor-mediated uptake of antibody-conjugated particles by dendritic cells as well as migration of particle-carrying dendritic cells to lymph nodes and stimulation of naïve T cells leading to enhanced cellular immune response as confirmed by specific cell lysis and IFN-{gamma} secretion.
acid-degradable particle, drug delivery, targeted vaccine
0027-8424
18264-18268
Kwon, Young Jik
c60cdda4-77b8-4239-9241-3b011d17e6ed
James, Edward
7dc1afb7-d326-4050-89fc-1f4e2a1a19a4
Shastri, Nilabh
8af29105-c59b-4260-bdde-a97c016e0793
Fréchet, Jean M.J.
90dd831c-4569-47cd-8ca4-aa7afe503604
Kwon, Young Jik
c60cdda4-77b8-4239-9241-3b011d17e6ed
James, Edward
7dc1afb7-d326-4050-89fc-1f4e2a1a19a4
Shastri, Nilabh
8af29105-c59b-4260-bdde-a97c016e0793
Fréchet, Jean M.J.
90dd831c-4569-47cd-8ca4-aa7afe503604

Kwon, Young Jik, James, Edward, Shastri, Nilabh and Fréchet, Jean M.J. (2005) In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles. Proceedings of the National Academy of Sciences of the United States of America, 102 (51), 18264-18268. (doi:10.1073/pnas.0509541102).

Record type: Article

Abstract

Activating the immune system to trigger a specific response is a major challenge in vaccine development. In particular, activating sufficient cytotoxic T lymphocyte-mediated cellular immunity, which is crucial for the treatment of many diseases including cancer and AIDS, has proven to be especially challenging. In this study, antigens were encapsulated in acid-degradable polymeric particle carriers to cascade cytotoxic T lymphocyte activation. To target dendritic cells, the most potent antigen-presenting cells, the particle carriers, were further conjugated with monoclonal antibodies. A series of ex vivo and in vivo studies have shown increased receptor-mediated uptake of antibody-conjugated particles by dendritic cells as well as migration of particle-carrying dendritic cells to lymph nodes and stimulation of naïve T cells leading to enhanced cellular immune response as confirmed by specific cell lysis and IFN-{gamma} secretion.

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More information

Published date: 2005
Keywords: acid-degradable particle, drug delivery, targeted vaccine
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 40675
URI: http://eprints.soton.ac.uk/id/eprint/40675
ISSN: 0027-8424
PURE UUID: 4c4533fc-c43c-406a-bc6d-ddb49956e850
ORCID for Edward James: ORCID iD orcid.org/0000-0001-8638-7928

Catalogue record

Date deposited: 07 Jul 2006
Last modified: 16 Mar 2024 03:51

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Contributors

Author: Young Jik Kwon
Author: Edward James ORCID iD
Author: Nilabh Shastri
Author: Jean M.J. Fréchet

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