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A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery

A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery
A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery
Microbubbles are currently in clinical use as ultrasound contrast agents and under active investigation as mediators of ultrasound therapy. To improve the theranostic potential of microbubbles, nanoparticles can be attached to the bubble shell for imaging, targeting and/or enhancement of acoustic response. Existing methods for fabricating particle-loaded bubbles, however, require the use of polymers, oil layers or chemical reactions for particle incorporation; embed/attach the particles that can reduce echogenicity; impair biocompatibility; and/or involve multiple processing steps. Here, we describe a simple method to embed nanoparticles in a phospholipid-coated microbubble formulation that overcomes these limitations. Magnetic nanoparticles are used to demonstrate the method with a range of different microbubble formulations. The size distribution and yield of microbubbles are shown to be unaffected by the addition of the particles. We further show that the microbubbles can be retained against flow using a permanent magnet, can be visualised by both ultrasound and magnetic resonance imaging (MRI) and can be used to transfect SH-SY5Y cells with fluorescent small interfering RNA under the application of a magnetic field and ultrasound field.
2190-3948
342-356
Owen, Joshua
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Crake, Calum
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Lee, Jeong Yu
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Carugo, Dario
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Beguin, Estelle
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Khrapichev, Alexandr
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Browning, Richard J.
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Sibson, Nicola
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Stride, Eleanor
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Owen, Joshua
4e7fc6bc-f2c5-4622-894f-9e811eca84cd
Crake, Calum
f1f0af19-23cd-4d50-ba45-6e7c8173c1e6
Lee, Jeong Yu
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Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Beguin, Estelle
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Khrapichev, Alexandr
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Browning, Richard J.
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Sibson, Nicola
56d18ace-6668-45fc-9240-eb0388493601
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Owen, Joshua, Crake, Calum, Lee, Jeong Yu, Carugo, Dario, Beguin, Estelle, Khrapichev, Alexandr, Browning, Richard J., Sibson, Nicola and Stride, Eleanor (2018) A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery. Drug Delivery and Translational Research, 8 (2), 342-356. (doi:10.1007/s13346-017-0366-7).

Record type: Article

Abstract

Microbubbles are currently in clinical use as ultrasound contrast agents and under active investigation as mediators of ultrasound therapy. To improve the theranostic potential of microbubbles, nanoparticles can be attached to the bubble shell for imaging, targeting and/or enhancement of acoustic response. Existing methods for fabricating particle-loaded bubbles, however, require the use of polymers, oil layers or chemical reactions for particle incorporation; embed/attach the particles that can reduce echogenicity; impair biocompatibility; and/or involve multiple processing steps. Here, we describe a simple method to embed nanoparticles in a phospholipid-coated microbubble formulation that overcomes these limitations. Magnetic nanoparticles are used to demonstrate the method with a range of different microbubble formulations. The size distribution and yield of microbubbles are shown to be unaffected by the addition of the particles. We further show that the microbubbles can be retained against flow using a permanent magnet, can be visualised by both ultrasound and magnetic resonance imaging (MRI) and can be used to transfect SH-SY5Y cells with fluorescent small interfering RNA under the application of a magnetic field and ultrasound field.

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JOwen-Drug Deliv and Transl Res-2017 - Version of Record
Available under License Creative Commons Attribution.
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Accepted/In Press date: 1 March 2017
e-pub ahead of print date: 15 March 2017
Published date: April 2018
Organisations: Bioengineering Group

Identifiers

Local EPrints ID: 406802
URI: http://eprints.soton.ac.uk/id/eprint/406802
ISSN: 2190-3948
PURE UUID: ebf79844-f93a-4e14-a78e-2550b4413f2c

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Date deposited: 22 Mar 2017 02:09
Last modified: 15 Mar 2024 12:27

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Contributors

Author: Joshua Owen
Author: Calum Crake
Author: Jeong Yu Lee
Author: Dario Carugo
Author: Estelle Beguin
Author: Alexandr Khrapichev
Author: Richard J. Browning
Author: Nicola Sibson
Author: Eleanor Stride

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