Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.
281-287
Hu, Chunxiao
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Kalsi, Sumit
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Zeimpekis, Ioannis
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Sun, Kai
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Ashburn, Peter
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Turner, Carrie
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Sutton, J. Mark
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Morgan, Hywel
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15 October 2017
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Kalsi, Sumit
0ec1f5c2-a6ec-4c07-92fb-15708c28742e
Zeimpekis, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
b7c648a3-7be8-4613-9d4d-1bf937fb487b
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Turner, Carrie
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Sutton, J. Mark
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hu, Chunxiao, Kalsi, Sumit, Zeimpekis, Ioannis, Sun, Kai, Ashburn, Peter, Turner, Carrie, Sutton, J. Mark and Morgan, Hywel
(2017)
Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors.
Biosensors & Bioelectronics, 96, .
(doi:10.1016/j.bios.2017.05.016).
Abstract
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.
Text
Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
- Accepted Manuscript
Text
1-s2.0-S0956566317303263-main
- Version of Record
More information
Accepted/In Press date: 8 May 2017
e-pub ahead of print date: 9 May 2017
Published date: 15 October 2017
Organisations:
Optoelectronics Research Centre, Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 408072
URI: http://eprints.soton.ac.uk/id/eprint/408072
ISSN: 0956-5663
PURE UUID: 3024968b-40d6-42fb-8c42-98abdc3f45b2
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Date deposited: 11 May 2017 01:03
Last modified: 16 Mar 2024 05:20
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Contributors
Author:
Chunxiao Hu
Author:
Sumit Kalsi
Author:
Carrie Turner
Author:
J. Mark Sutton
Author:
Hywel Morgan
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