Oligonucleotide-conjugated thiazole orange probes as “light-up” probes for messenger ribonucleic acid molecules in living cells
Privat, Eric, Melvin, Tracy, Asseline, Ulysse and Vigny, Paul (2001) Oligonucleotide-conjugated thiazole orange probes as “light-up” probes for messenger ribonucleic acid molecules in living cells. Photochemistry and Photobiology, 74, (4), 532-541. (doi:10.1562/0031-8655(2001)074<0532:OCTOPA>2.0.CO;2).
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Description/Abstract
“Light-up” probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-α-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.
| Item Type: | Article |
|---|---|
| ISSNs: | 0031-8655 (print) |
| Related URLs: | |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > Optoelectronics Research Centre |
| Item ID: | 41236 |
| Date Deposited: | 04 Aug 2006 |
| Last Modified: | 21 Nov 2012 17:08 |
| Contributors: | Privat, Eric (Author) Melvin, Tracy (Author) Asseline, Ulysse (Author) Vigny, Paul (Author) |
| Date: | October 2001 |
| Status: | Published |
| Contact Email Address: | tm@orc.soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/41236 |
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