Oligonucleotide-conjugated thiazole orange probes as “light-up” probes for messenger ribonucleic acid molecules in living cells


Privat, Eric, Melvin, Tracy, Asseline, Ulysse and Vigny, Paul (2001) Oligonucleotide-conjugated thiazole orange probes as “light-up” probes for messenger ribonucleic acid molecules in living cells. Photochemistry and Photobiology, 74, (4), 532-541. (doi:10.1562/0031-8655(2001)074<0532:OCTOPA>2.0.CO;2).

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Description/Abstract

“Light-up” probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-α-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.

Item Type: Article
ISSNs: 0031-8655 (print)
Related URLs:
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > Optoelectronics Research Centre
ePrint ID: 41236
Date Deposited: 04 Aug 2006
Last Modified: 27 Mar 2014 18:26
Contact Email Address: tm@orc.soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/41236

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