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Taking tissue engineering principles into theatre: augmentation of impacted allograft with human bone marrow stromal cells

Taking tissue engineering principles into theatre: augmentation of impacted allograft with human bone marrow stromal cells
Taking tissue engineering principles into theatre: augmentation of impacted allograft with human bone marrow stromal cells
Human bone marrow contains bone progenitor cells that arise from multipotent mesenchymal stem cells. Seeding bone progenitor cells onto a scaffold can produce a 3D living composite with significant mechanical and biological potential. This article details laboratory and clinical findings from two clinical cases, where different proximal femoral conditions were treated using impacted allograft augmented with marrow-derived autogenous progenitor cells. Autologous bone marrow was seeded onto highly washed morselized allograft and impacted. Samples of the impacted graft were also taken for ex vivo analysis. Both patients made an uncomplicated clinical recovery. Imaging confirmed defect filling with encouraging initial graft incorporation. Histochemical and alkaline phosphatase staining demonstrated that a live composite graft with osteogenic activity had been introduced into the defects. These studies demonstrate that marrow-derived cells can adhere to highly washed morselized allograft, survive the impaction process and proliferate with an osteoblastic phenotype, thus creating a living composite.
bone marrow aspirate, human allograft, impaction bone grafting
1746-0751
685-692
Tilley, Simon
fc6eeb2f-ffb5-4cc9-9565-c7c40175c284
Bolland, Benjamin J.R.F.
0f256518-5316-4376-bb6a-e5eff75fda71
Partridge, Kris
64fd1f95-bf24-4251-ad6e-4c96068b7fc5
New, Andrew M.R.
d2fbaf80-3abd-4bc5-ae36-9c77dfdde0d6
Latham, Jeremy M.
c63671a2-248f-489f-b620-64ff19bc20f5
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Tilley, Simon
fc6eeb2f-ffb5-4cc9-9565-c7c40175c284
Bolland, Benjamin J.R.F.
0f256518-5316-4376-bb6a-e5eff75fda71
Partridge, Kris
64fd1f95-bf24-4251-ad6e-4c96068b7fc5
New, Andrew M.R.
d2fbaf80-3abd-4bc5-ae36-9c77dfdde0d6
Latham, Jeremy M.
c63671a2-248f-489f-b620-64ff19bc20f5
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Tilley, Simon, Bolland, Benjamin J.R.F., Partridge, Kris, New, Andrew M.R., Latham, Jeremy M., Dunlop, Douglas G. and Oreffo, Richard O.C. (2006) Taking tissue engineering principles into theatre: augmentation of impacted allograft with human bone marrow stromal cells. Regenerative Medicine, 1 (5), 685-692. (doi:10.2217/17460751.1.5.685).

Record type: Article

Abstract

Human bone marrow contains bone progenitor cells that arise from multipotent mesenchymal stem cells. Seeding bone progenitor cells onto a scaffold can produce a 3D living composite with significant mechanical and biological potential. This article details laboratory and clinical findings from two clinical cases, where different proximal femoral conditions were treated using impacted allograft augmented with marrow-derived autogenous progenitor cells. Autologous bone marrow was seeded onto highly washed morselized allograft and impacted. Samples of the impacted graft were also taken for ex vivo analysis. Both patients made an uncomplicated clinical recovery. Imaging confirmed defect filling with encouraging initial graft incorporation. Histochemical and alkaline phosphatase staining demonstrated that a live composite graft with osteogenic activity had been introduced into the defects. These studies demonstrate that marrow-derived cells can adhere to highly washed morselized allograft, survive the impaction process and proliferate with an osteoblastic phenotype, thus creating a living composite.

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More information

Published date: September 2006
Keywords: bone marrow aspirate, human allograft, impaction bone grafting

Identifiers

Local EPrints ID: 42990
URI: http://eprints.soton.ac.uk/id/eprint/42990
ISSN: 1746-0751
PURE UUID: e01411e9-a4d1-46d5-a591-41a78f67cfe2
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 08 Jan 2007
Last modified: 16 Mar 2024 03:11

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Contributors

Author: Simon Tilley
Author: Benjamin J.R.F. Bolland
Author: Kris Partridge
Author: Andrew M.R. New
Author: Jeremy M. Latham
Author: Douglas G. Dunlop

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