Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase


Millar, Timothy M. (2004) Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase. FEBS Letters, 562, (1-3), 129-133. (doi:10.1016/S0014-5793(04)00218-2).

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Description/Abstract

One electron reductions of oxygen and nitrite by xanthine oxidase form peroxynitrite. The nitrite and oxygen reducing activities of xanthine oxidase are regulated by oxygen with K(oxygen) 26 and 100 microM and K(nitrite) 1.0 and 1.1 mM with xanthine and NADH as donor substrates. Optimal peroxynitrite formation occurs at 70 microM oxygen with purine substrates. Kinetic parameters: V(max) approximately 50 nmol/min/mg and K(m) of 22, 36 and 70 microM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.

Item Type: Article
ISSNs: 0014-5793 (print)
Related URLs:
Keywords: xanthine oxidase, peroxynitrite, nitric oxide, superoxide
Subjects: Q Science > QR Microbiology
Divisions: University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
ePrint ID: 44319
Date Deposited: 23 Feb 2007
Last Modified: 27 Mar 2014 18:28
URI: http://eprints.soton.ac.uk/id/eprint/44319

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