DNA triple-helix formation at target sites containing duplex mismatches

Rusling, David A., Brown, Tom and Fox, Keith R. (2006) DNA triple-helix formation at target sites containing duplex mismatches. Biophysical Chemistry, 123, (2-3), 134-140. (doi:10.1016/j.bpc.2006.04.016).


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We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N = bis-amino-U (BAU) (for stable recognition of AT base pairs) and N = S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A. CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.

Item Type: Article
ISSNs: 0301-4622 (print)
Related URLs:
Keywords: triple helix, mismatch, thermal melting forming oligonucleotides, base-pairs, physiological ph, recognition, motif, nucleoside, guanine, ta, cg
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 44536
Date Deposited: 07 Mar 2007
Last Modified: 06 Aug 2015 02:37
URI: http://eprints.soton.ac.uk/id/eprint/44536

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