DNA triple-helix formation at target sites containing duplex mismatches
Rusling, David A., Brown, Tom and Fox, Keith R. (2006) DNA triple-helix formation at target sites containing duplex mismatches. Biophysical Chemistry, 123, (2-3), 134-140. (doi:10.1016/j.bpc.2006.04.016).
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We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N = bis-amino-U (BAU) (for stable recognition of AT base pairs) and N = S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A. CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.
|Keywords:||triple helix, mismatch, thermal melting forming oligonucleotides, base-pairs, physiological ph, recognition, motif, nucleoside, guanine, ta, cg|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Chemistry
|Date Deposited:||07 Mar 2007|
|Last Modified:||01 Jun 2011 02:26|
|Contributors:||Rusling, David A. (Author)
Brown, Tom (Author)
Fox, Keith R. (Author)
|Contact Email Address:||K.R.Fox@soton.ac.uk|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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