Phospholamban and sarcolipin are maintained in the endoplasmic reticulum by retrieval from the ER-Golgi intermediate compartment


Butler, John, Lee, Anthony G., Wilson, David I., Spalluto, Cosma, Hanley, Neil A. and East, J. Malcolm (2007) Phospholamban and sarcolipin are maintained in the endoplasmic reticulum by retrieval from the ER-Golgi intermediate compartment. Cardiovascular Research, 74, (1), 114-123. (doi:10.1016/j.cardiores.2007.01.006).

Download

Full text not available from this repository.

Description/Abstract

Objective

Phospholamban and sarcolipin are small transmembrane proteins that modulate cardiac contractility through their interaction with the sarcoplasmic reticulum (SR) calcium pumps (SERCAs). We have examined the hypothesis that phospholamban and sarcolipin are maintained in the SR by a process of retrieval from post-SR compartments and the role of their transmembrane domains in targeting.

Methods

Antibodies directed against phospholamban and protein markers of the endoplasmic reticulum/Golgi intermediate compartment (ERGIC) and the trans-Golgi were used in fluorescence microscopy studies of cultured human fetal cardiac myocytes. In addition, sarcolipin and phospholamban were tagged at the N-terminus with enhanced-green-fluorescent protein (EGFP) and expressed in COS 7 cells. The EGFP-tagged constructs were localised using fluorescence microscopy and cell fractionation. The length of the transmembrane domains of phospholamban and sarcolipin were extended and the effect on cellular location was also examined.

Results

In fetal cardiac myocytes phospholamban was located in the SR and the ERGIC, but did not migrate to the trans-Golgi network. Tagged-phospholamban and sarcolipin were located in the endoplasmic reticulum (ER) of COS 7 cells indicating that their targeting was unaffected by the EGFP tag. Significant proportions of the tagged phospholamban and sarcolipin were also located in the ERGIC but not in the trans-Golgi. Increasing the length of the transmembranous domains of EGFP-tagged phospholamban and sarcolipin resulted in their mis-targeting to the plasma membrane.

Conclusions

Phospholamban and sarcolipin are maintained in the SR/ER by a process that includes their retrieval from the ERGIC following their passage from the SR/ER into the ERGIC. The transmembrane domains of phospholamban and sarcolipin are involved in the retrieval process.

Item Type: Article
Additional Information: This paper describes the way in which the regulators of endoplasmic reticulum (ER) calcium pumps, phospholamban and sarcolipin, are maintained in the ER and prevented from entering the plasma membrane.
ISSNs: 0008-6363 (print)
Related URLs:
Keywords: phospholamban, sarcolipin, targetting, endoplasmic reticulum, sarcoplasmic reticulum, retrieval, ERGIC, transmembrane domain
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 44691
Date Deposited: 16 Apr 2007
Last Modified: 27 Mar 2014 18:29
URI: http://eprints.soton.ac.uk/id/eprint/44691

Actions (login required)

View Item View Item