Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens
Vaughan, Thomas E., Skipp, Paul J., O'Connor, C. David, Hudson, Michael J., Vipond, Richard, Elmore, Michael J. and Gorringe, Andrew R. (2006) Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens. Vaccine, 24, (25), 5277-5293. (doi:10.1016/j.vaccine.2006.03.013).
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Vaccines to prevent meningococcal disease have been developed from the outer membrane vesicles (OMVs) of Neisseria meningitidis and the related commensal organism Neisseria lactamica. In addition to lipopolysaccharide and the major porins, these vaccines contain a large number of proteins that are incompletely characterised. Here we describe comparative proteomic analyses of the N. lactamica OMV vaccine and OMVs from a serogroup B strain of N. meningitidis. Tandem mass-spectrometry data for trypsinised N. lactamica OMV vaccine were matched to an incompletely assembled genome sequence from the same strain to give 65 robust protein identifications and a further 122 single- or two-peptide matches. Fifty-seven N. meningitidis K454 proteins were identified robustly (and a further 68 from single- or two-peptide matches) by inference from the N. meningitidis MC58 genome. The results suggest that OMVs have a hitherto unappreciated complexity and pinpoint novel candidate antigens for further characterisation.
|Keywords:||neisseria meningitidis, neisseria lactamica, vaccine, outer membrane vesicle, proteomics, tandem mass spectrometry|
|Subjects:||R Medicine > RB Pathology
Q Science > QR Microbiology > QR180 Immunology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||09 Mar 2007|
|Last Modified:||27 Mar 2014 18:29|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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