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AUX1 promotes lateral root formation by facilitating indole-3-acetic acid distribution between sink and source tissues in the arabidopsis seedling

AUX1 promotes lateral root formation by facilitating indole-3-acetic acid distribution between sink and source tissues in the arabidopsis seedling
AUX1 promotes lateral root formation by facilitating indole-3-acetic acid distribution between sink and source tissues in the arabidopsis seedling
Arabidopsis root architecture is regulated by shoot-derived signals such as nitrate and auxin. We report that mutations in the putative auxin influx carrier AUX1 modify root architecture as a result of the disruption in hormone transport be- tween indole-3-acetic acid (IAA) source and sink tissues. Gas chromatography–selected reaction monitoring–mass spectrometry measurements revealed that the aux1 mutant exhibited altered IAA distribution in young leaf and root tis- sues, the major IAA source and sink organs, respectively, in the developing seedling. Expression studies using the auxin-inducible reporter IAA2 :: uidA revealed that AUX1 facilitates IAA loading into the leaf vascular transport system. AUX1 also facilitates IAA unloading in the primary root apex and developing lateral root primordium. Exogenous appli- cation of the synthetic auxin 1-naphthylacetic acid is able to rescue the aux1 lateral root phenotype, implying that root auxin levels are suboptimal for lateral root primordium initiation in the mutant.
1040-4651
589-597
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Bhalerao, Rishikesh
e414e4c1-53dd-4c1f-8a32-93a8ecf71b1c
Casimiro, Ilda
bb668bc2-bf37-4d44-8dc1-7a771f497bf1
Eklof, Jan
683699e6-2ab1-4c18-abd7-9e9105c8fb95
Casero, Pedro J
27e375d5-3df2-43c9-8e1a-0181d8973834
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c
Sandberg, Goran
5807cf0b-1e87-47d5-bd3f-d67f6ac83bf9
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Bhalerao, Rishikesh
e414e4c1-53dd-4c1f-8a32-93a8ecf71b1c
Casimiro, Ilda
bb668bc2-bf37-4d44-8dc1-7a771f497bf1
Eklof, Jan
683699e6-2ab1-4c18-abd7-9e9105c8fb95
Casero, Pedro J
27e375d5-3df2-43c9-8e1a-0181d8973834
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c
Sandberg, Goran
5807cf0b-1e87-47d5-bd3f-d67f6ac83bf9

Marchant, Alan, Bhalerao, Rishikesh, Casimiro, Ilda, Eklof, Jan, Casero, Pedro J, Bennett, Malcolm and Sandberg, Goran (2002) AUX1 promotes lateral root formation by facilitating indole-3-acetic acid distribution between sink and source tissues in the arabidopsis seedling. The Plant Cell, 14 (3), 589-597. (doi:10.1105/tpc.010354).

Record type: Article

Abstract

Arabidopsis root architecture is regulated by shoot-derived signals such as nitrate and auxin. We report that mutations in the putative auxin influx carrier AUX1 modify root architecture as a result of the disruption in hormone transport be- tween indole-3-acetic acid (IAA) source and sink tissues. Gas chromatography–selected reaction monitoring–mass spectrometry measurements revealed that the aux1 mutant exhibited altered IAA distribution in young leaf and root tis- sues, the major IAA source and sink organs, respectively, in the developing seedling. Expression studies using the auxin-inducible reporter IAA2 :: uidA revealed that AUX1 facilitates IAA loading into the leaf vascular transport system. AUX1 also facilitates IAA unloading in the primary root apex and developing lateral root primordium. Exogenous appli- cation of the synthetic auxin 1-naphthylacetic acid is able to rescue the aux1 lateral root phenotype, implying that root auxin levels are suboptimal for lateral root primordium initiation in the mutant.

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Submitted date: 14 August 2001
Published date: March 2002

Identifiers

Local EPrints ID: 45438
URI: http://eprints.soton.ac.uk/id/eprint/45438
ISSN: 1040-4651
PURE UUID: 3ef5c668-9410-4816-b937-06efd55a9154

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Date deposited: 03 Apr 2007
Last modified: 15 Mar 2024 09:11

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Contributors

Author: Alan Marchant
Author: Rishikesh Bhalerao
Author: Ilda Casimiro
Author: Jan Eklof
Author: Pedro J Casero
Author: Malcolm Bennett
Author: Goran Sandberg

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