Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues
Ubeda-Tomas, Susana, Edvardsson, Ellinor, Eland, Cathlene, Singh, Kumar, Zadik, Daniel, Aspeborg, Henrik, Gorzsas, Andras, Teeri, Tuula T., Sundberg, Bjorn, Persson, Per, Bennett, Malcolm and Marchant, Alan (2007) Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues. Physiologia Plantarum, 129, (2), 415-428. (doi:10.1111/j.1399-3054.2006.00817.x).
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Despite the importance of secondary growth in plants, relatively few genes
regulating this process have been identified to date. By using data from detailed
transcript profiling of the poplar wood-forming tissues, 150 genes that are
differentially expressed within the zone of secondary growth were identified.
In order to determine the possible function of these poplar genes, potential
Arabidopsis thaliana orthologs were identified and gene knockout lines anal-
ysed. Three selection filters were used to identify the most likely orthologous
genes using poplar and Arabidopsis sequence comparisons, expression pro-
filing in secondary thickened Arabidopsis hypocotyls and global expression
analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740),
the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no
proposed function AtUNKA (At4g27435) were selected for further detailed
analysis of their role in secondary growth in Arabidopsis. The presented
genome-based approach using both poplar and Arabidopsis systems provides
powerful means towards assigning biological functions to enzymes with
poorly understood biochemical activity, such as AtCSLA2 and AtGUT1, as
well as for proteins with no known function.
|Subjects:||S Agriculture > SD Forestry
S Agriculture > SB Plant culture
Q Science > QH Natural history > QH301 Biology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||29 Mar 2007|
|Last Modified:||01 Jun 2011 06:55|
|Contact Email Address:||firstname.lastname@example.org|
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