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Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues

Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues
Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues
Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar wood-forming tissues, 150 genes that are differentially expressed within the zone of secondary growth were identified. In order to determine the possible function of these poplar genes, potential Arabidopsis thaliana orthologs were identified and gene knockout lines anal- ysed. Three selection filters were used to identify the most likely orthologous genes using poplar and Arabidopsis sequence comparisons, expression pro- filing in secondary thickened Arabidopsis hypocotyls and global expression analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740), the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no proposed function AtUNKA (At4g27435) were selected for further detailed analysis of their role in secondary growth in Arabidopsis. The presented genome-based approach using both poplar and Arabidopsis systems provides powerful means towards assigning biological functions to enzymes with poorly understood biochemical activity, such as AtCSLA2 and AtGUT1, as well as for proteins with no known function.
0031-9317
415-428
Ubeda-Tomas, Susana
624dc8b2-dc9e-4e88-a3cf-af0b7d558b9e
Edvardsson, Ellinor
40515989-d046-44b0-a695-f0aaeea0b1d9
Eland, Cathlene
9b35c49f-e227-4267-9013-bacc829eb340
Singh, Kumar
aebe6474-1f25-44ae-80f4-614cf30d4788
Zadik, Daniel
ff36375c-a3ee-4972-8fb8-7855c7a09217
Aspeborg, Henrik
96f31bb7-447d-4c49-99ea-e80b7de80aae
Gorzsas, Andras
4421ca64-0595-4c60-928a-6d40667d55d5
Teeri, Tuula T.
6c433a9d-ff15-40f0-8602-0208e781b4ff
Sundberg, Bjorn
f46fa385-a598-481b-8397-f413cdbb5bc5
Persson, Per
78579c88-5a38-48f0-8aec-4fe5e1e1ea40
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Ubeda-Tomas, Susana
624dc8b2-dc9e-4e88-a3cf-af0b7d558b9e
Edvardsson, Ellinor
40515989-d046-44b0-a695-f0aaeea0b1d9
Eland, Cathlene
9b35c49f-e227-4267-9013-bacc829eb340
Singh, Kumar
aebe6474-1f25-44ae-80f4-614cf30d4788
Zadik, Daniel
ff36375c-a3ee-4972-8fb8-7855c7a09217
Aspeborg, Henrik
96f31bb7-447d-4c49-99ea-e80b7de80aae
Gorzsas, Andras
4421ca64-0595-4c60-928a-6d40667d55d5
Teeri, Tuula T.
6c433a9d-ff15-40f0-8602-0208e781b4ff
Sundberg, Bjorn
f46fa385-a598-481b-8397-f413cdbb5bc5
Persson, Per
78579c88-5a38-48f0-8aec-4fe5e1e1ea40
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e

Ubeda-Tomas, Susana, Edvardsson, Ellinor, Eland, Cathlene, Singh, Kumar, Zadik, Daniel, Aspeborg, Henrik, Gorzsas, Andras, Teeri, Tuula T., Sundberg, Bjorn, Persson, Per, Bennett, Malcolm and Marchant, Alan (2007) Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues. Physiologia Plantarum, 129 (2), 415-428. (doi:10.1111/j.1399-3054.2006.00817.x).

Record type: Article

Abstract

Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar wood-forming tissues, 150 genes that are differentially expressed within the zone of secondary growth were identified. In order to determine the possible function of these poplar genes, potential Arabidopsis thaliana orthologs were identified and gene knockout lines anal- ysed. Three selection filters were used to identify the most likely orthologous genes using poplar and Arabidopsis sequence comparisons, expression pro- filing in secondary thickened Arabidopsis hypocotyls and global expression analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740), the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no proposed function AtUNKA (At4g27435) were selected for further detailed analysis of their role in secondary growth in Arabidopsis. The presented genome-based approach using both poplar and Arabidopsis systems provides powerful means towards assigning biological functions to enzymes with poorly understood biochemical activity, such as AtCSLA2 and AtGUT1, as well as for proteins with no known function.

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Published date: 2007

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Local EPrints ID: 45439
URI: http://eprints.soton.ac.uk/id/eprint/45439
ISSN: 0031-9317
PURE UUID: 7fbf99b3-f8bd-4741-bcd5-4f00340fec59

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Date deposited: 29 Mar 2007
Last modified: 15 Mar 2024 09:11

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Contributors

Author: Susana Ubeda-Tomas
Author: Ellinor Edvardsson
Author: Cathlene Eland
Author: Kumar Singh
Author: Daniel Zadik
Author: Henrik Aspeborg
Author: Andras Gorzsas
Author: Tuula T. Teeri
Author: Bjorn Sundberg
Author: Per Persson
Author: Malcolm Bennett
Author: Alan Marchant

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