Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells
Bateman, Elizabeth Alice Louise, Ardern-Jones, Michael Roger and Ogg, Graham Stuart (2006) Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. Journal of Allergy and Clinical Immunology, 118, (6), 1350-1356. (doi:10.1016/j.jaci.2006.07.040).
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Description/Abstract
Background: although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. Objective: to determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. Methods: using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. Conclusion: circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. Clinical implications: persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease.
| Item Type: | Article |
|---|---|
| ISSNs: | 0091-6749 (print) |
| Related URLs: | |
| Keywords: | T cells, atopic dermatitis, memory T cells, Fel d 1 |
| Subjects: | R Medicine > RL Dermatology Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair |
| Item ID: | 47940 |
| Date Deposited: | 15 Aug 2007 |
| Last Modified: | 01 Jun 2011 07:00 |
| Contributors: | Bateman, Elizabeth Alice Louise (Author) Ardern-Jones, Michael Roger (Author) Ogg, Graham Stuart (Author) |
| Date: | December 2006 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/47940 |
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