Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells
Bateman, Elizabeth Alice Louise, Ardern-Jones, Michael Roger and Ogg, Graham Stuart (2006) Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. Journal of Allergy and Clinical Immunology, 118, (6), 1350-1356. (doi:10.1016/j.jaci.2006.07.040).
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Background: although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. Objective: to determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. Methods: using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. Conclusion: circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. Clinical implications: persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease.
|Keywords:||T cells, atopic dermatitis, memory T cells, Fel d 1|
|Subjects:||R Medicine > RL Dermatology
Q Science > QH Natural history > QH301 Biology
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
|Date Deposited:||15 Aug 2007|
|Last Modified:||27 Mar 2014 18:31|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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