A microfluidic based system for analysis of single cells based on Ca2+ flux
A microfluidic based system for analysis of single cells based on Ca2+ flux
A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.
5093-5100
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Cooper, Jon M.
2dbda23a-e372-4d05-baf1-48b243ced44f
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
November 2006
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Cooper, Jon M.
2dbda23a-e372-4d05-baf1-48b243ced44f
Haswell, Stephen J.
443a65de-9f13-4fbf-8b70-7de24004957b
Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J.
(2006)
A microfluidic based system for analysis of single cells based on Ca2+ flux.
Electrophoresis, 27 (24), .
(doi:10.1002/elps.200600390).
Abstract
A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.
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Published date: November 2006
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Local EPrints ID: 49139
URI: http://eprints.soton.ac.uk/id/eprint/49139
ISSN: 0173-0835
PURE UUID: 51162727-37da-42d3-ade8-2dcf3888e8a0
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Date deposited: 24 Oct 2007
Last modified: 16 Mar 2024 03:55
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Author:
Huabing Yin
Author:
Jon M. Cooper
Author:
Stephen J. Haswell
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