Novel fluorescent proteins for high-content screening


Wolff, M., Wiedenmann, J., Nienhaus, G.U., Valler, M. and Heilker, R. (2006) Novel fluorescent proteins for high-content screening. Drug Discovery Today, 11, (23-24), 1054-1060. (doi:10.1016/j.drudis.2006.09.005).

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Description/Abstract

The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.

Item Type: Article
ISSNs: 1359-6446 (print)
Related URLs:
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
ePrint ID: 51168
Date Deposited: 08 May 2008
Last Modified: 27 Mar 2014 18:34
URI: http://eprints.soton.ac.uk/id/eprint/51168

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