Novel fluorescent proteins for high-content screening
Wolff, M., Wiedenmann, J., Nienhaus, G.U., Valler, M. and Heilker, R. (2006) Novel fluorescent proteins for high-content screening. Drug Discovery Today, 11, (23-24), 1054-1060. (doi:10.1016/j.drudis.2006.09.005).
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Description/Abstract
The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.
| Item Type: | Article |
|---|---|
| ISSNs: | 1359-6446 (print) |
| Related URLs: | |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES) |
| Item ID: | 51168 |
| Date Deposited: | 08 May 2008 |
| Last Modified: | 01 Jun 2011 00:25 |
| Contributors: | Wolff, M. (Author) Wiedenmann, J. (Author) Nienhaus, G.U. (Author) Valler, M. (Author) Heilker, R. (Author) |
| Date: | December 2006 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/51168 |
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