Diffractive micro bar codes for encoding of biomolecules in multiplexed assays.


Broder, Graham R., Ranasinghe, Rohan T., She, Joseph K., Banu, Shahanara, Birtwell, Sam W., Cavalli, Gabriel, Galitonov, Gerasim S., Holmes, David, Martins, Hugo F.P., MacDonald, Kevin F., Neylon, Cameron, Zheludev, Nikolay, Roach, Peter L. and Morgan, Hywel (2008) Diffractive micro bar codes for encoding of biomolecules in multiplexed assays. Analytical Chemistry, 80, (6), 1902-1909. (doi:10.1021/ac7018574 S0003-2700(70)01857-7).

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Description/Abstract

Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in <45 min. Analysis of raw data from assays carried out on the diffractive microparticles indicates that the reproducibility and sensitivity approach those of commercial encoding platforms. Micrometer-sized particles, imprinted with several superimposed diffraction gratings, can encode many million unique codes. The high encoding capacity of this technology along with the applicability of the manufactured bar codes to multiplexed assays will allow accurate measurement of a wide variety of molecular interactions, leading to new opportunities in diverse areas of biotechnology such as genomics, proteomics, high-throughput screening, and medical diagnostics.

Item Type: Article
ISSNs: 0003-2700 (print)
Related URLs:
Subjects: Q Science > QR Microbiology
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TP Chemical technology
Divisions: University Structure - Pre August 2011 > School of Electronics and Computer Science
University Structure - Pre August 2011 > Optoelectronics Research Centre
University Structure - Pre August 2011 > School of Physics and Astronomy
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 52037
Date Deposited: 09 Jun 2008
Last Modified: 27 Mar 2014 18:35
URI: http://eprints.soton.ac.uk/id/eprint/52037

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