Antimicrobial-resistance of Streptococcus pneumoniae isolated from the Lebanese environment

Harakeh, Steve, Yassine, Hadi and El-Fadel, Mutasem (2006) Antimicrobial-resistance of Streptococcus pneumoniae isolated from the Lebanese environment. Marine Environmental Research, 62, (3), 181-193. (doi:10.1016/j.marenvres.2006.03.010).


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The aim of this study was to isolate and molecularly characterize Streptococcus pneumoniae in samples collected from the aquatic environment in Lebanon. For this purpose, 57 samples of ground waters, sea water, sediments and crabs were collected. Biochemical tests as well as PCR were used for further identification and molecular characterization of 46 suspected isolates using specific primers for the lytA-gene and protein 2B-gene. The confirmed isolates were evaluated for their antimicrobial resistance, using the minimum inhibitory concentration method. Crab samples exhibited a high contamination level of an average count of 8.2 × 104 CFU/g. In water samples, the highest contamination was recorded near a coastal landfill with a bacterial count of 1.8 × 103 CFU/ml whereas the lowest bacterial counts (non detectable) were observed in ground water samples. Twenty out of the 46 suspected isolates came from sea water, and 17 out of those were characterized by PCR as S. pneumoniae. Testing the antimicrobial resistance of the 20 isolates to various antimicrobials showed that all sea water isolates were resistant to vancomycin, gentamicin, and oxacillin (100%), whereas 62.5% were resistant to clindamycin, and only 25% were erythromycin resistant. Resistance rates were higher in the case of fresh water samples, with 57% resistance to erythromycin, and 100% resistance to vancomycin, gentamicin, clindamycin and oxacillin. Alternatively, all tested crab isolates were susceptible to erythromycin. However, they showed a high resistance of 80% against vancomycin, gentamicin, clindamycin and 100% against oxacillin

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.marenvres.2006.03.010
ISSNs: 0141-1136 (print)
Related URLs:
Keywords: streptococcus pneumoniae, antimicrobial resistance, marine environment, fresh water environment, molecular characterization
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Q Science > QR Microbiology
Divisions : University Structure - Pre August 2011 > School of Civil Engineering and the Environment
ePrint ID: 52903
Accepted Date and Publication Date:
September 2006Published
Date Deposited: 15 Jul 2008
Last Modified: 31 Mar 2016 12:31

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