Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions


Li, Hong, Broughton-Head, Victoria J., Fox, Keith R. and Brown, Tom (2007) Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions. Nucleosides, Nucleotides and Nucleic Acids, 26, (8 & 9), 1005-1009. (doi:10.1080/15257770701508554).

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Description/Abstract

Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and 5-(3-aminoprop-2-ynyl)-'-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.

Item Type: Article
ISSNs: 1525-7770 (print)
Related URLs:
Keywords: psoralen, triplex, nucleosides, 2-aminopyridine, crosslinking
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 54336
Date Deposited: 30 Jul 2008
Last Modified: 27 Mar 2014 18:37
URI: http://eprints.soton.ac.uk/id/eprint/54336

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