Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos
Hwang, Gyulin, Muller, Ferenc, Rahman, M. Aziz, Williams, Darren W., Murdock, Paul J., Pasi, K. John, Goldspink, Geoffrey, Farahmand, Hamid and Maclean, Norman (2004) Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos. Marine Biotechnology, 6, (5), 485-492. (doi:10.1007/s10126-004-3121-2).
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A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
|Keywords:||human coagulation factor VII, transgenic fish, bioreactor|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||05 Aug 2008|
|Last Modified:||06 Aug 2015 02:44|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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