Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes

Vannahme, C., Smyth, N., Miosge, N., Gosling, S., Frie, C., Paulsson, M., Maurer, P. and Hartmann, U. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes. Journal of Biological Chemistry, 277, (41), 37977-37986. (doi:10.1074/jbc.M203830200).


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We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1074/jbc.M203830200
ISSNs: 0021-9258 (print)
Related URLs:
Subjects: Q Science > Q Science (General)
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 55856
Accepted Date and Publication Date:
1 October 2002Published
Date Deposited: 06 Aug 2008
Last Modified: 31 Mar 2016 12:36

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