Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes

Vannahme, C., Smyth, N., Miosge, N., Gosling, S., Frie, C., Paulsson, M., Maurer, P. and Hartmann, U. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes. The Journal of Biological Chemistry, 277 (41), 37977-37986. (doi:10.1074/jbc.M203830200).

Abstract

We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.

This record has no associated files available for download.

Contributors

Download statistics