Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes
Vannahme, C., Smyth, N., Miosge, N., Gosling, S., Frie, C., Paulsson, M., Maurer, P. and Hartmann, U. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes. Journal of Biological Chemistry, 277, (41), 37977-37986. (doi:10.1074/jbc.M203830200).
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Description/Abstract
We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.
| Item Type: | Article |
|---|---|
| ISSNs: | 0021-9258 (print) |
| Related URLs: | |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 55856 |
| Date Deposited: | 06 Aug 2008 |
| Last Modified: | 01 Jun 2011 04:57 |
| Contributors: | Vannahme, C. (Author) Smyth, N. (Author) Miosge, N. (Author) Gosling, S. (Author) Frie, C. (Author) Paulsson, M. (Author) Maurer, P. (Author) Hartmann, U. (Author) |
| Date: | 1 October 2002 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/55856 |
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