Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium
Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium
We have examined the stability of fluorescently-labelled oligonucleotides that are based on the human telomeric repeat [(GGGTTA)3GGG], in which one of the guanines in turn is substituted with inosine. We show that the relative stability of the substitutions is different in the presence of sodium and potassium. The data for potassium suggest a parallel arrangement of the strands, while the sodium form is mixed parallel and antiparallel.
Graphical abstract
The relative stability of intramolecular quadruplexes containing inosine substitutions is different in sodium and potassium.
G-quartet, quadruplex, fluorescence, molecular beacon, DNA melting, inosine
2047-2050
Risitano, A.
cf59ac40-0ade-4e18-ab7e-354b4026b8a3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f
15 April 2005
Risitano, A.
cf59ac40-0ade-4e18-ab7e-354b4026b8a3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f
Risitano, A. and Fox, K.R.
(2005)
Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium.
Bioorganic & Medicinal Chemistry Letters, 15 (8), .
(doi:10.1016/j.bmcl.2005.02.050).
Abstract
We have examined the stability of fluorescently-labelled oligonucleotides that are based on the human telomeric repeat [(GGGTTA)3GGG], in which one of the guanines in turn is substituted with inosine. We show that the relative stability of the substitutions is different in the presence of sodium and potassium. The data for potassium suggest a parallel arrangement of the strands, while the sodium form is mixed parallel and antiparallel.
Graphical abstract
The relative stability of intramolecular quadruplexes containing inosine substitutions is different in sodium and potassium.
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Published date: 15 April 2005
Keywords:
G-quartet, quadruplex, fluorescence, molecular beacon, DNA melting, inosine
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Local EPrints ID: 55887
URI: http://eprints.soton.ac.uk/id/eprint/55887
PURE UUID: 6a1cc652-04e3-4201-8c22-abbc06f351ac
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Date deposited: 06 Aug 2008
Last modified: 16 Mar 2024 02:36
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Author:
A. Risitano
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