Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum
Fotopoulos, V., Holmes, R., Hall, J.L. and Williams, L.E. (2006) Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum. Mycological Research, 110, (1), 28-37. (doi:10.1016/j.mycres.2005.08.011).
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Description/Abstract
Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.
| Item Type: | Article |
|---|---|
| ISSNs: | 0953-7562 (print) |
| Related URLs: | |
| Keywords: | arabidopsis thaliana, erysiphales, plant pathology, plasma membrane H+-ATPase |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 55897 |
| Date Deposited: | 06 Aug 2008 |
| Last Modified: | 12 May 2013 01:08 |
| Contributors: | Fotopoulos, V. (Author) Holmes, R. (Author) Hall, J.L. (Author) Williams, L.E. (Author) |
| Date: | 1 January 2006 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/55897 |
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