Actions of glutamate and ivermectin on the pharyngeal muscle of Ascaridia galli: A comparative study with Caenorhabditis elegans

Holden-Dye, Linda and Walker, Robert J. (2006) Actions of glutamate and ivermectin on the pharyngeal muscle of Ascaridia galli: A comparative study with Caenorhabditis elegans. International Journal for Parasitology, 36, (4), 395-402. (doi:10.1016/j.ijpara.2005.11.006).


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The actions of glutamate and ivermectin were examined in the pharynx of Ascaridia galli and the results compared with those on the pharynx of Caenorhabditis elegans. In both preparations glutamate elicits a depolarization and inhibition of pharyngeal pumping, but the response of the pharynx of A. galli was much less than for C. elegans. This may be either because the pharyngeal membrane potential of the former is closely linked to the equilibrium potential for chloride ions (ECl) while that of C. elegans is independent of ECl, or that there is a lower density of glutamate receptors on the pharyngeal muscle of A. galli compared with C. elegans. The maximum depolarization to glutamate of the pharyngeal muscle was 4.5±0.8 mV in A. galli while it was >25 mV in C. elegans. Picrotoxin was a weak antagonist of the glutamate response in both species. Flufenamic acid, pentobarbitone and flurazepam had no significant effect on either preparation at concentrations up to 100 ?M. Three glutamate receptor agonists, ibotenate, kainate and quisqualate were all more potent than glutamate on the A. galli pharyngeal muscle. In contrast, only ibotenate was more potent than glutamate in C. elegans pharynx, the other two agonists being approximately 20 times less potent. The potency of ivermectin differed markedly between the two species, being approximately three orders of magnitude less potent on the pharynx of A. galli compared with C. elegans. This study demonstrates clear differences between the properties of the pharyngeal muscle of the two species and shows that care must be taken when extrapolating data from free-living to parasitic species of nematode.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.ijpara.2005.11.006
ISSNs: 0020-7519 (print)
1879-0135 (electronic)
Related URLs:
Keywords: ascaridia galli, ivermectin, caenorhabitis elegans, electrophysiology, pharynx
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 55941
Accepted Date and Publication Date:
1 April 2006Published
Date Deposited: 06 Aug 2008
Last Modified: 31 Mar 2016 12:36

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