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Brush border structure defects in I-plastin deficient mice

Brush border structure defects in I-plastin deficient mice
Brush border structure defects in I-plastin deficient mice
Plastin (or fimbrin) is an actin-bundling protein consisting of two actin-binding domains in tandem preceded by two calcium-binding EF-hands. In mammals three different isoforms are expressed in a cell-type specific manner. Iplastinis specifically expressed in the small intestine, colon and kidney, where it localizes in microvilli, specialized surface structures with highly ordered microfilament bundles. We have generated knockout mice lacking I-plastin. I-plastin deficient mice showed no overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies specific for T- and L-plastin were used to demonstrate that lacking of I-plastin is not compensated by another plastin isoform. Transmission electron microscopy studies revealed morphological differences between wild type andI-plastin deficient mice in the microvilli. Brush border preparations of knockout mice are sensitive and seem to degrade easily. In immunofluorescence studies of intestinal mucosa cryosections decreased actin staining of the apical membrane was observed. Another abundant actin cross-linker of the brush border, villin, was found to be absent from the apical membrane. I-plastin seems to be an important regulator of the morphological structure and stability of microvilli.
0171-9335
p.22
Grimm-Günter, Eva-Maria S.
975968cb-bf33-424f-b949-035ff9e0b757
Ramos, Sonia
c531d816-634e-4bc0-a3a9-7af2d630e08b
Smyth, Neil
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Celine, Revenu
a56dbf7c-fb22-471e-a601-f97ca2a4081f
Hurbain, Ilse
b3583e80-2831-47e0-9692-78e4bc830528
Raposo, Graca
d9a66afd-c77d-4afa-9e96-498b35cd5255
Robine, Sylvie
98edf452-68bc-45ea-902f-b38f6a7e63fc
Rivero, Francisco
5fa8e3a1-f222-44ac-9dd5-7cba281d50fb
Grimm-Günter, Eva-Maria S.
975968cb-bf33-424f-b949-035ff9e0b757
Ramos, Sonia
c531d816-634e-4bc0-a3a9-7af2d630e08b
Smyth, Neil
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Celine, Revenu
a56dbf7c-fb22-471e-a601-f97ca2a4081f
Hurbain, Ilse
b3583e80-2831-47e0-9692-78e4bc830528
Raposo, Graca
d9a66afd-c77d-4afa-9e96-498b35cd5255
Robine, Sylvie
98edf452-68bc-45ea-902f-b38f6a7e63fc
Rivero, Francisco
5fa8e3a1-f222-44ac-9dd5-7cba281d50fb

Grimm-Günter, Eva-Maria S., Ramos, Sonia, Smyth, Neil, Celine, Revenu, Hurbain, Ilse, Raposo, Graca, Robine, Sylvie and Rivero, Francisco (2006) Brush border structure defects in I-plastin deficient mice. European Journal of Cell Biology, 85 (Supplement 1), p.22. (doi:10.1016/j.ejcb.2006.02.011).

Record type: Article

Abstract

Plastin (or fimbrin) is an actin-bundling protein consisting of two actin-binding domains in tandem preceded by two calcium-binding EF-hands. In mammals three different isoforms are expressed in a cell-type specific manner. Iplastinis specifically expressed in the small intestine, colon and kidney, where it localizes in microvilli, specialized surface structures with highly ordered microfilament bundles. We have generated knockout mice lacking I-plastin. I-plastin deficient mice showed no overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies specific for T- and L-plastin were used to demonstrate that lacking of I-plastin is not compensated by another plastin isoform. Transmission electron microscopy studies revealed morphological differences between wild type andI-plastin deficient mice in the microvilli. Brush border preparations of knockout mice are sensitive and seem to degrade easily. In immunofluorescence studies of intestinal mucosa cryosections decreased actin staining of the apical membrane was observed. Another abundant actin cross-linker of the brush border, villin, was found to be absent from the apical membrane. I-plastin seems to be an important regulator of the morphological structure and stability of microvilli.

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Published date: 1 March 2006

Identifiers

Local EPrints ID: 55963
URI: http://eprints.soton.ac.uk/id/eprint/55963
ISSN: 0171-9335
PURE UUID: e8cc8279-3845-4bd2-92e2-b1b68894e45d

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Date deposited: 17 Jul 2009
Last modified: 15 Mar 2024 10:58

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Contributors

Author: Eva-Maria S. Grimm-Günter
Author: Sonia Ramos
Author: Neil Smyth
Author: Revenu Celine
Author: Ilse Hurbain
Author: Graca Raposo
Author: Sylvie Robine
Author: Francisco Rivero

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