Brush border structure defects in I-plastin deficient mice

Grimm-Günter, Eva-Maria S., Ramos, Sonia, Smyth, Neil, Celine, Revenu, Hurbain, Ilse, Raposo, Graca, Robine, Sylvie and Rivero, Francisco (2006) Brush border structure defects in I-plastin deficient mice. European Journal of Cell Biology, 85, (Supplement 1), p.22. (doi:10.1016/j.ejcb.2006.02.011).


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Plastin (or fi mbrin) is an actin-bundling protein consisting
of two actin-binding domains in tandem preceded by two
calcium-binding EF-hands. In mammals three different
isoforms are expressed in a cell-type specifi c manner. Iplastin
is specifi cally expressed in the small intestine,
colon and kidney, where it localizes in microvilli,
specialized surface structures with highly ordered
microfi lament bundles. We have generated knockout
mice lacking I-plastin. I-plastin defi cient mice showed no
overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies
specifi c for T- and L-plastin were used to demonstrate
that lacking of I-plastin is not compensated by another
plastin isoform. Transmission electron microscopy studies
revealed morphological differences between wildtype and
I-plastin defi cient mice in the microvilli. Brush border
preparations of knockout mice are sensitive and seem
to degrade easily. In immunofl uorescence studies of
intestinal mucosa cryosections decreased actin staining
of the apical membrane was observed. Another abundant
actin cross-linker of the brush border, villin, was found to
be absent from the apical membrane. I-plastin seems to be
an important regulator of the morphological structure and
stability of microvilli.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.ejcb.2006.02.011
ISSNs: 0171-9335 (print)
Related URLs:
Subjects: Q Science > QP Physiology
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 55963
Accepted Date and Publication Date:
1 March 2006Published
Date Deposited: 17 Jul 2009
Last Modified: 31 Mar 2016 12:36

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