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Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site

Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site
Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site
The translation initiation factor eIF4A is cleaved within mammalian cells infected by foot-and-mouth disease virus (FMDV). The FMDV 3C protease cleaves eIF4AI (between residues E143 and V144), but not the closely related eIF4AII. Modification of eIF4AI, to produce a sequence identical to eIF4AII around the cleavage site, blocked proteolysis. Alignment of mammalian eIF4AI onto the three-dimensional structure of yeast eIF4A located the scissile bond within an exposed, flexible portion of the molecule. The N- and C-terminal cleavage products of eIF4AI generated by FMDV 3C dissociate. Cleavage of eIF4AI by FMDV 3C is thus expected to inactivate it.
picornavirus, translation initiation factor eIF4A, protein synthesis, 3C protease, foot-and-mouth disease virus
0014-5793
1-5
Lin, Wei
d7b04e93-7fa4-4442-b6d6-622099d52f27
Ross-Smith, Natalie
a689f539-3ce4-4777-ad42-6ece168c8e32
Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3
Belsham, Graham J.
f1f1c89b-b63a-4066-a41e-65ee08d4b56b
Avila, Jesus (ed.)
0b4536e1-10b1-43aa-a3d7-c48b5ab128c2
Lin, Wei
d7b04e93-7fa4-4442-b6d6-622099d52f27
Ross-Smith, Natalie
a689f539-3ce4-4777-ad42-6ece168c8e32
Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3
Belsham, Graham J.
f1f1c89b-b63a-4066-a41e-65ee08d4b56b
Avila, Jesus (ed.)
0b4536e1-10b1-43aa-a3d7-c48b5ab128c2

Lin, Wei, Ross-Smith, Natalie, Proud, Christopher G., Belsham, Graham J. and Avila, Jesus (ed.) (2001) Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site. FEBS Letters, 507 (1), 1-5.

Record type: Article

Abstract

The translation initiation factor eIF4A is cleaved within mammalian cells infected by foot-and-mouth disease virus (FMDV). The FMDV 3C protease cleaves eIF4AI (between residues E143 and V144), but not the closely related eIF4AII. Modification of eIF4AI, to produce a sequence identical to eIF4AII around the cleavage site, blocked proteolysis. Alignment of mammalian eIF4AI onto the three-dimensional structure of yeast eIF4A located the scissile bond within an exposed, flexible portion of the molecule. The N- and C-terminal cleavage products of eIF4AI generated by FMDV 3C dissociate. Cleavage of eIF4AI by FMDV 3C is thus expected to inactivate it.

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More information

Published date: 19 October 2001
Keywords: picornavirus, translation initiation factor eIF4A, protein synthesis, 3C protease, foot-and-mouth disease virus

Identifiers

Local EPrints ID: 55964
URI: http://eprints.soton.ac.uk/id/eprint/55964
ISSN: 0014-5793
PURE UUID: eb6e47f2-bca7-4bf6-9930-a8ccb2588658

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 08 Jan 2022 13:04

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Contributors

Author: Wei Lin
Author: Natalie Ross-Smith
Author: Christopher G. Proud
Author: Graham J. Belsham
Author: Jesus (ed.) Avila

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