A dedicated translation factor controls the synthesis of the global regulator Fis

Owens, Róisín M., Pritchard, Gareth, Skipp, Paul, Hodey, Michelle, Connell, Sean R., Nierhaus, Knud H. and Connor, C. David (2004) A dedicated translation factor controls the synthesis of the global regulator Fis. The EMBO Journal, 23, (16), 3375-3385. (doi:10.1038/sj.emboj.7600343).


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Original Publication URL: http://dx.doi.org/10.1038/sj.emboj.7600343


BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the 'fast-track' expression of specific mRNAs may have wider significance.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1038/sj.emboj.7600343
ISSNs: 0261-4189 (print)
Related URLs:
Keywords: Fis, GTPase, regulation, ribosome, translation
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 55972
Accepted Date and Publication Date:
1 August 2004Published
Date Deposited: 07 Aug 2008
Last Modified: 31 Mar 2016 12:36
URI: http://eprints.soton.ac.uk/id/eprint/55972

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